OFFICIAL INQUIRY FILES and DOCUMENTS
PORTUGUESE FORENSIC INSTITUTE TESTS

This information belongs to the Ministério Público in Portimão, Portugal.
It was released to the public on 4 August 2008 in accordance with Portuguese Law

836 Preliminary directions for lab analysis 2007.05.09
Report of laboratory analysis of vestiges from 5A
04-Processos Vol IV Page 836
also Outros Apensos 1, Vol I Page 4

TRANSLATIONS ALBYM

04_VOLUME_IVa_Page_836

To: The Coordinator of the Criminal Investigation


Date: 2007/05/09

Subject: Laboratory Analysis of the samples gathered with regard to the disappearance of the child Madeleine McCann.

Dear Sir,

In accordance with the request made in your fax no. 388 of 8th May 2007, the samples collected by officers from the Local Crime Scene of the LPC concerning the examination of Apartment 5A of the OC in P da L, were delivered to the Genetics and Biological Forensics Service of the INML - Lisbon delegation, in order for the respective laboratory analyses to be carried out,.

According to the information provided by Dra Ribeiro, from the previously mentioned Institute, the analysis of the samples will begin from today, however the urgent delivery of mouth swabs, containing the biological samples of each of the parents and the siblings is necessary as well as those of the tourist group who were on holiday with them.

I hereby request the urgent delivery of the mouth swabs referred to as well as a letter requesting the implementation of the laboratory analyses, in order to be able to carry them out, this letter should be sent to the Genetics and Forensic Biology Service of the INML, Fax****.
With compliments.
Director of the LPC.
1713  Letter 5 June 2007 requesting analysis of Marks & Spencer pyjamas, 2-3 year old, 97 cm

 TRANSLATED BY INES

07 Processos Vol VII Page 1713
07_VOLUME_VI1a_Page_1713

To: Police Scientific Laboratory
Lisbon

5th June 2007

Subject: Sending of Pyjamas

The present inquiry investigates the disappearance of Madeleine McCann on 3rd May 2007. I am herewith delivering to the Police Scientific Laboratory a pair of girl's pyjamas.

The Pyjamas are from Marks and Spencers, size 2 to 3 years -97 cm.

The pyjamas are composed of two pieces: camisole type without buttons and half sleeves, pink with designs, letters and tracing in white with (small) floral patterns, the right pyjama bottom leg has a design (smaller size) which is identical to that of the camisole.

The pyjamas being sent are 'equal' in make, model, size, colours and designs as well as presumably the texture, to those the little girl was wearing at the time of her disappearance. The article sent serves for eventual comparisons with fibres collected by the competent officers of the Police Scientific Lab, within the scope of the current investigation.

With compliments

Signed

The Coordinator of the Criminal Investigation

Goncalo Amaral

1723 to 1725  Letter 15 March 2007 from LPC to PJ Portimao asking what should be done with the pyjamas there having been no fibres collected during the Inquiry
07 Processos Vol VII Page 1723 to 1725
07_VOLUME_VI1a_Page_1723
Letter
To: The Coordinator of the Criminal Investigation

Date: 2007/03/15 (sic)

Ref: NUIPC 201/07 GALGS

Your communication: 2007/06/05

Ref no. 15971 Reg Correspondence 6429/07

Subject: Information

With reference to the abovementioned letter and in compliance with the despatch, we request you to provide us with information with regard to what should be done with the material sent, given that in this Scientific Police Laboratory there are no fibres that have been collected within the scope of the investigation mentioned above.
With compliments.

PP The Director of the SPL

Armando Santos
(Haed of Sector)
07 Processos Vol VII Page 1724
 also Outros Apensos I, Vol I Page 33
07_VOLUME_VI1a_Page_1724
Letter

To: Scientific Police Laboratory

Date: 5th June 2007

Subject: Sending of Pyjamas

The present inquiry investigates the disappearance of Madeleine McCann on 3rd May 2007 from P da L.

By means of this note I am sending a set of child's pyjamas to the Scientific Police Laboratory.

The pyjamas are of Marks $ Spencers make and size 2/3 years, 97 cm.

The pyjamas are composed of two pieces, a camisole without buttons and half length sleeves, pink in colour with designs and letters and white trousers with floral motifs (small), on the right leg there is a design (smaller size) that is the same as the design on the camisole.

The pyjamas being sent are 'equal' in make, model, size, colours and designs and presumably texture, as those the girl was wearing at the moment of her disappearance.

The article sent serves for eventual comparison with 'fibres' collected during the competent examinations carried out by staff from the SPL, within this inquiry.

Compliments.

G. Amaral

(Note: there is handwriting over the text which I can't fully make out).
 
07 Processos Vol II Page 1725
 also Outros Apensos I, Vol I Page 34
07_VOLUME_VI1a_Page_1725
Handwritten)

Examinations Carried Out

BG - 200708360

8359
8357
7143

CR/L - 200708272

8271
8270
8269
8268
7357
7356
7116
7060
Total - 13 examinations
1810 to 1816 - Cover letter and Police Scientific Laboratory Report 31 May 2007 re: Quinta dos Figos forensic recoveries 2007.05.06
Processos Vol VII Pages 1810 to 1816
also Outros Apensos Vol I Pages 37 to 46
07_VOLUME_VI1a_Page_1810
07_VOLUME_VI1a_Page_1811
07_VOLUME_VI1a_Page_1812
07_VOLUME_VI1a_Page_1813
07_VOLUME_VI1a_Page_1814
07_VOLUME_VI1a_Page_1815
07_VOLUME_VI1a_Page_1816


Scientific Police Laboratory Report

Forensic Report No. 200707116-CR/L




Requesting Party: Portimao DIC of the Policia Judiciaria

NUIPC: 201/070 GALGS

Request number: 189/2007

Examination initiated on 06 May 2007


The report on the collection of blood vestiges carried out on 06 May 2007, from the wall, next to the main entrance of Quinta dos Figos, situated on Rua 1 de Maio, in Praia da Luz, in Lagos, is requested.

Observations and Analyses Made

A team from the Crime Scene section of the Scientific Police Laboratory, composed of the undersigned officers, after a request made by the Portimao DIC of the Policia Judiciaria, went to Rua 1 de Maio, in Praia da Luz, in Lagos, where we were requested to proceed to collect various stains, supposedly haematic that were on the wall and the ground in that street. This collection was within the area of investigation into the disappearance of an English girl.

Here is a map of the scene.

Photo 1: Map of the scene showing the entrance to Quinta dos Figos encircled in red.


1811.jpg

Upon the team's arrival at the scene it was reported to us by the inspectors present that the supposed blood stains were found on a wall and on the ground close to the wall, next to main entrance of Quinta dos Figos

Photo 2 : Panoramic view of the site where the supposed blood stains were found.

Photos 3 , 4 and 5: Entrance to the Quinta dos Figos where eventual biological vestiges were found and detail of the plaque with the name of the property and of the plaque with the street name.

1812.jpg


Photo 6: Shot of the area of the Quinta dos Figos wall where the supposed blood stains were found.

From the observation made of the wall and its proximities various stains were detected with a brownish red colour which suggested that they could be of haematic origin. These were submitted to tests to detect peroxide existing in the blood using the Kastle-Mayer test, in both cases the reactions were positive.

The stain referred to as vestige no. 3 was further submitted for testing to determine whether the blood was of animal origin, but reacted positively as being human blood.

The blood stains mentioned previously were separated by zones and referenced as vestige no. 1 those being the stains found on the gravel ground, vestiges no. 2 being the blood stains that were found on top of the wall and vestige no. 3 as being the stains that were found on the ground in the area where there cobblestones.


1813.jpg


Photo 7: Shot of vestiges 1, 2 and 3.

Photos 8 and 9: Detail of vestige 1

1814.jpg


Photos 10 and 11: Detail of vestige 2.

Photos 12 and 13: Detail of vestige 3

Photo 14: Indicator test of the presence of human blood

1815.jpg


The collection of the vestiges indicated as vestiges nos. 1, 2 and 3 was begun using slightly moistened swabs, in accordance with the procedures of the Scientific Police Laboratory's Biology Area.

Conclusion:

Upon the request of the Portimao DIC present at the site, collection was made of 3 swabs containing blood vestiges, referred to in this report as vestiges nos. 1,2 and 3, which were delivered to the Biology Area, by means of delivery guide no. 189/2007 - CR/L, copy of which is annexed, in order to be preserved for further/eventual forensic examinations.

The test for the determination of animal species carried out on vestige 3 was placed in an evidence bag series A no. 022917 and delivered to the Portimao DIC.

Lisbon, 31st May 2007.

Signed.

1816.jpg

1817 - Delivery Note of Vestiges Recovered at Crime Scene  2007.05.06
07 Processos Vol VII Page 1817
07_VOLUME_VI1a_Page_1817
 

Delivery Guide No. 189/2007 - CR/L




Date of Delivery: 24 May 2007

Destination: Scientific Police Laboratory, Biology Area

Forensic Examination no.: 200707116-CR/L

NUIPC: 201/07.o GALGS

Date and Time of Collection: Wall of the Quinta dos Figos, Rua 1 de Maio, Praia da Luz

Scope of the test: Disappearance of Girl.

Requested by: Chief Inspector Tavares de Almeida, Portimão DIC.


Vestiges Delivered:

No. Description

1 Cotton swab containing blood vestiges collected from the ground next to the wall.
2 Cotton swab containing blood vestiges collected from the top of the wall.
3 Cotton swab containing blood vestiges collected from the ground next to the wall.

Observations: Nothing to be registered.

Lisbon, 25th May 2007

Delivery and receipt signed.

1818 - Term of embedment regarding collected vestige 2007.06.12
07 Processos Vol VII Page 1818
07_VOLUME_VI1a_Page_1818

Policia Judiciara

NUIPC-201 070 GALGS
4th Brigade
Inspector Joao Carlos



Terms Of Withdrawal


I consign to the case files that I proceeded to the withdrawal of vestige no. 3 (evidence bag no. 022917) to which the previous report refers, with the aim of depositing it later.

Conclusion


Portimao
12th June 2007


Signed
Inspector Joao Carlos

1826 E-mail from F. Corte Real to Luis Neves
1827 Report/List of mitochondrial DNA profiles re: disappearance (191 samples)
1828 Consent of Maria Irene Trovao Ferro for DNA analysis of mouth swab/hair sample
07-Processos Vol VII Page 1826-1828
07_VOLUME_VIIa_Page_1826
07_VOLUME_VIIa_Page_1827
07_VOLUME_VIIa_Page_1828
From: D Corte, INML
To: Luis Neves

Date: 19th June 2007

Dear Dr Luis,

I enclose the data from the analysis of all the samples. We will repeat some that did not give results.

With compliments

F. Corte Real



---------------------

Processos Vol VII Page 1827

Profiles of mitochondrial DNA from the samples sent






---------------------

Processos Vol VII Page 1828

Consent of Maria Irene Trovao Ferro for DNA analysis of mouth swab/hair sample
2211 - Fax to the Police Scientific Laboratory regarding vestiges found
2212 - Confirmation above fax sent

TRANSLATED BY INES

08 Processos Vol VIII Page 2211
08_VOLUME_VIIIa_Page_2211
08_VOLUME_VIIIa_Page_2212


Fax

Date: 10th August 2007

To: The Scientific Police Laboratory, "Crime Scene"

From: Portimao DIC

NUIPC 201/07 GALGS

This fax is to confirm the requests made to the team of this Crime Scene laboratory for the carrying out of inspections (already done) of the places and objects indicated on these days:

1. 01 August 2007

Place: Apartment 5 A Garden (front) apartment space
OC resort

Diligence: detection of biological vestiges and others.

2. 04 August 2007.

Place: Apartment 5 A
Ocean Club resort

Diligence: detection of biological vestiges and others.

3. 06 August 2007

Place: Portimao car park - 1st May square
Object: vehicle. Numberplate 59-DA-27
Make/model: Renault Scenic

With compliments

Chief Inspector

Tavares de Almeida

2213 to 2214 - Fax to Police Scientific Laboratory regarding other vestiges

TRANSLATION BY INES

08 Processos Vol VIII Pages 2213 - 2214
08_VOLUME_VIIIa_Page_2213
 
08_VOLUME_VIIIa_Page_2214
 

Fax

Urgent

Ref: 640

Date: 13 August 2007

To: Scientific Police Laboratory. Crime Scene.

Addendum to Fax 638, 10th August

In addendum to our fax mentioned above we inform you of the means of treatment to be given to the vestiges/articles collected.

01 August 2007

Conditioned and sent to British laboratory (accompanied by the SPL).
1. Mosaics from the floor of the living room of apartment 5 A, OC.
2. Bushes from the garden of apartment 5 A, OC.

Conditioned and to preserved at SPL:
3. Fibres.
4. Hairs.

04 August 2007

Conditioned and sent to British laboratory (accompanied by the SPL).
5. Vestiges, eventually biological.

06 August 2007

Conditioned and sent to British laboratory (accompanied by the SPL).
6. Vestiges, eventually biological.
7. Vestiges/fibres.

With compliments

Chief Inspector

Tavares de Almeida

PT text:

2303 to 2304 - List of OC employees who have been asked for mouth swab and hair sample

TRANSLATED BY INES

Processos Vol IX Pages 2303 to  2304
09_VOLUME_IXa_Page_2303
09_VOLUME_IXa_Page_2304

Policia Judiciaria
INML Instituto Nacional de Medicina Legal
Lisbon Delegation

Urgent
Date: 04-07-2007

Subject: Material for Laboratory Examination – Sending of Mouth Swabs and Hair Samples.

Within the current investigation into the disappearance of Madeleine McCann, which occurred on 3rd May 2007 in Praia da Luz, Lagos,

Collection of samples from the following persons are being sent.

- Silvia Maria Correia Ramos Batista – Hair sample
- Mário Domingos Moreira – Mouth swab and hair sample.
- Luís Filipe Monteiro Ferro – Mouth swab and hair sample.
- Dina María dos Reis Rocha – Mouth swab and hair sample
- Marina Paula Ramos Batista Castela – Mouth swab and hair sample
- Maria Júlia Serafim da Silva – Mouth swab and hair sample
- José María Batista Roque- Mouth swab and hair sample
- José Leal Pimental – Mouth swab and hair sample
- António Conceicao Duarte: Mouth swab and hair sample
- Nelson Filipe Pacheco da Costa – Mouth swab and hair sample
- Maria Irene Trovao Ferro – Mouth swab and hair sample
- Joao Trigo Barreiras – Mouth swab and hair sample
- Vitor Manuel Martins – Mouth swab

This letter and the accompanying material was delivered by hand.

Signed

Chief Inspector
Tavares de Almeida

2383 to 2393 Police Scientific report (dated 9 July) of exams and vestiges collected from an apartment in Burgau on 5 May 2007
09 Processos Vol IX Pages 2381 to 2393
also Outros Apensos Vol I Pages 186 to 198
09_VOLUME_IXa_Page_2381
09_VOLUME_IXa_Page_2382
09_VOLUME_IXa_Page_2383
09_VOLUME_IXa_Page_2384
09_VOLUME_IXa_Page_2385
09_VOLUME_IXa_Page_2386
09_VOLUME_IXa_Page_2387
09_VOLUME_IXa_Page_2388
09_VOLUME_IXa_Page_2389
09_VOLUME_IXa_Page_2390
09_VOLUME_IXa_Page_2391
09_VOLUME_IXa_Page_2392
09_VOLUME_IXa_Page_2393

Report of Forensic Examination
No. 200707356 CR/L



Requesting Party: Portimao DIC

NUIPC: 201/07.0 GALGS

Fax No. 414 of 15th May 2007



Examination initiated on : 05 May 2007


Report of forensic examination carried out in an apartment situated in Aparthotel Sol e Mar, Apartment 2C, Burgau, with regard to the disappearance of an English girl.

Observations and Analyses Made


On 5th May 2007 at about 19.30 a team from the Scientific Police Laboratory Local Crime Scene, composed of those undersigned, upon the request of the Policia Judiciaria, went to an apartment situated in the Aparthotel Sol e Mar, 2nd floor, Apartment C in Burgau to carry out forensic examinations of the site.

Photos of the exterior of the apartment to be examined follow as well as details of its entrance.

Photo 1:

Panoramic view of Aparthotel Sol e Mar, a red arrow indicates the entrance door to the apartment subject to examination.


2383.jpg

Photos 2 and 3:

Panoramic views of the ascending and descending access road to Aparthotal Sol e Mar, photo 2 showing by means of a red arrow the external access stairs to the different apartments of the Aparthotel.

Photo 4 and 5:

Shot of the external access stairs to the Aparthotel and the external access corridor that exists on the second floor, the entrance to the apartment subject to examination is shown by means of a red arrow.

2384.jpg

Photos 6 and 7:

Shot of the entrance door to the apartment subject to examination and detail of the letter 'C' on the door.

Initially it was proceeded to make a first general observation of the apartment, having verified that it was made up of a kitchen, living room, bathroom and bedroom.

This was followed by a detailed observation of the interior of the apartment with the aim of proceeding to search and collect forensic vestiges relevant to this examination.

Initially it was proceeded to search for shoe prints or a child's foot prints, but no relevant vestiges were detected for carrying out this examination.

This was followed by a search for biological vestiges, fibres etc., inside the apartment subject to examination.

During this search several hairs were collected from the following parts of the apartment:


2385.jpg

- Various hairs were collected from the kitchen floor, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 1B.

- Various hairs were collected from the entrance corridor, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 2.

- Various hairs were collected from the bedroom floor, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 3.

- Various hairs were collected from on top of the bed in the bedroom, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 4.

- Various hairs were collected from the bathroom, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 5.

- Various hairs were collected from the living room floor, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 6.

- Various hairs were collected from a sofa on the right side of the living room, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 7

- Various hairs were collected from a sofa on the left side of the living room, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 8.


2386.jpg

The search for eventual haematic vestiges resulted in the detection of various brownish red stains that appeared to be of haematic origin, these were submitted to tests to search for the presence of peroxide existing in blood by means of the Kastle-Mayer test, only one stain, situated on a cupboard door in the kitchen reacted positively.

The stain in question was submitted to testing to determine the animal species of the blood and reacted positively as being human blood.

Given the results obtained it was proceeded to collect the haematic stain, by means of a slightly moistened cotton swab and this vestige was referenced as vestige 1 A.

Photo 8: Detail of the test for the determination of animal species.

The search carried out in the interior of the apartment did not detect the presence of other types of biological vestiges or fibres of interest to the forensic examination.

Finally, observation and search for haematic vestiges inside the apartment was made using a chemical product to search for latent haematic vestiges. In the application of this product no results characteristic of haematic vestiges were found.

Photos 9 and 10:

Detail of the entrance corridor from which various hairs referenced as vestige 2 were collected.

2387.jpg


Photos 11 - 13:

Interior of the kitchen and detail of area from which the hairs referenced as vestige IB were collected.

Photos 14 and 15:

Detail of the kitchen cupboard door from which the haematic stain referenced as 1 A was collected.


2388.jpg

Photos 16 - 18:
Detail of the bedroom from which various hairs were collected (floor) referenced as vestige 3.

Photos 19 - 20:
Detail of the bed area from which various hairs were collected referenced as vestige 4.

2389.jpg

Photos 21- 23:

Details of the bathroom from which various hairs were collected referenced as vestige 5.

Photos 24 and 25:

Inside of the living room from where various hairs were collected referenced as vestige 6.
2390.jpg

Photos 26 - 28:
Interior of the living room from which various hairs were collected referenced as vestige 6.

Photos 29 and 30:
The two sofas inside the living room on the right and on the left, from which various hairs were collected referenced as vestiges 7 (left) and 8 (right).

2391.jpg


Conclusions

From the forensic examination carried out on 5th May 2007 in an apartment in Aparthotel Sol e Mar, 2nd floor, Apartment C in Burgau, the following vestiges were collected:

- An haematic stain from the door of a cupboard in the kitchen, this vestige was collected using slightly moistened cotton swab, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 1 A.

-
- Various hairs were collected from the kitchen floor, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 1B.

- Various hairs were collected from the entrance corridor, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 2.

- Various hairs were collected from the bedroom floor, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 3.

- Various hairs were collected from on top of the bed in the bedroom, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 4.

- Various hairs were collected from the bathroom, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 5.

- Various hairs were collected from the living room floor, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 6.

- Various hairs were collected from a sofa on the right side of the living room, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 7

- Various hairs were collected from a sofa on the left side of the living room, they were placed in a paper envelope, according to the instructions of the Scientific Police Laboratory's Biology Area and were referenced as vestige no. 8.


In accordance with the request of the Portimao DIC the previously described vestiges, referenced from no. 1 to 8 were delivered to the Biology Area of the Scientific Police Laboratory, by means of delivery guide no. 196/2007 - CR/L, a copy is attached, to be preserved there for the carrying out of eventual future laboratory examinations.

The test for the determination of animal species applied to vestige 1 A was placed in evidence bag series A no. 034205 and remitted to the Portimao DIC.

Lisbon, 9th July 2007
 

2394 - Delivery report (dated 29 May 2007) of swab and hair collected from Burgau apartment.
09 Processos Vol IX Page 2394
09_VOLUME_IXa_Page_2394

Delivery Guide No. 196/2007 CR/L
Delivery Guide of Vestiges Collected at the Crime Scene

Date of Delivery: 29th May 2007
Destination: Biology Area of the Scientific Police Laboratory

Forensic Examination no.: 200707356-CR/L
NUIPC: 201/07.0 GALGS

Date and Time of Collection: 05.22.00 May 07
Place of Collection: Aparthotel Sol e Mar, 2nd floor, Apartment C, Burgau

Scope: Disappearance of Girl
Requested by: Chief Inspector Tavares de Almeida, Portimao DIC.

Vestiges Delivered:
No. Description
1A Cotton swab containing haematic vestiges collected from the kitchen cupboard door.
IB Various hairs collected from the kitchen floor.
2 Various hairs collected from the entrance corridor
3 Various hairs collected from the bedroom floor
4 Various hairs collected from the bed in the bedroom.
5 Various hairs collected from the bathroom.
6 Various hairs collected from the living room floor.
7 Various hairs collected from a sofa in the living room.
8 Various hairs collected from a sofa in the living room.

Lisbon 29th May 2007
Signed: delivery and receipt.

2416 to 2421 Letter dated 17 August to the National Institute for Legal Medicine with questions re: DNA; with response dated 21 August; and pages of mitochondrial test results from vestiges collected

TRANSLATION BY ALBYM

09-Processos Vol IX Page 2416-2421
09_VOLUME_IXa_Page_2416
09_VOLUME_IXa_Page_2417
09_VOLUME_IXa_Page_2418
09_VOLUME_IXa_Page_2419
09_VOLUME_IXa_Page_2420
09_VOLUME_IXa_Page_2421

Letter to the National Forensics Institute (INML) from Goncalo Amaral


17th August 2007

The present inquiry is in relation to the investigation of the disappearance of Madeleine McCann on 3rd May from P da L.

The English authorities in possession of elements collected from the family home in Leicestershire, which are being examined in an unidentified laboratory, traced the girl's DNA profile, in annex to this document.

As it is necessary to the investigation we request the following:

1. Whether in the tests done by the INML Madeleine's profile was established?

2. In the case of a positive answer to point 1, that it should be determined whether the girl is the daughter of the parents identified ? Gerald Patrick McCann and Kate Healy.

3. Information about whether the profile obtained by the INML corresponds to the profile traced in the English laboratory, on the request of the British authorities.

4. If there are differences between the English and Portuguese profiles that a report should be drawn up for this inquiry.

5. In the follow-up of point 4, we request to be informed whether in the English profile the girl is the daughter of the McCann couple.

6. Other information that might be of use to the investigation.

Signed G. Amaral

09-Processos Vol IX Page 2419

Vol IX Page 2419

Reply from the Forensics Institute (INML) to Goncalo Amaral


22-08-2007

Reply to Queries

- We inform you that none of the samples received by this institute were designated as supposedly belonging to the missing girl and we therefore, cannot reply to this query.


- Samples were studied - hair and a piece of cloth - nuclear DNA profiles only being obtained from 4 samples, which upon comparison with the DNA profiles of Kate and Gerry, could not belong to the girl.

- The samples were then studied using mitochondrial DNA analysis, the same was done for the other samples, giving the results in accordance with our report of 9th July No. 2007/000226 LX-BC.

- As requested in point 5, it was determined that the profile obtained by the British lab could belong to a son/daughter of the McCanns.

- The comparison of the profiles obtained in autossomic STR from Kate and Gerry McCann with the profile obtained was carried out.
Signed

2422 to 2439 Report from INML dated 9 July 2007, with correspondence showing that it took 3 weeks to reach Portimao (on 31 July) from the PJ National Directorate in Lisbon as follows:
--> 2425 - 11 July from INML to National Directorate, Lisbon
--> 2424 - 17 July from ND, Lisbon to Alipio Ribeiro, personally, in Lisbon
--> 2423 - 20 July from Alipio Ribeiro to PJ Regional office, Faro
--> 2422 - 26 July from Faro to Portimao
09-Processos Vol IX Page 2422-2439
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09_VOLUME_IXa_Page_2439
FIRST INML REPORT 9 JULY 2007

 
NATIONAL INSTITUTE OF LEGAL MEDICINE
GENETIC AND BIOLOGICAL FORENSIC SERVICES
  Processo no. 2007/000268/P-B
Processo no. 2007/000244/CR-B
Processo no. 2007/000226/LX-BC
 
EXPERT REPORT

IDENTIFICATION OF BIOLOGICAL TRACES AND COMPARATIVE STUDY WITH REFERENCE SAMPLES

INFORMATION


REQUESTING AGENCY:
Departamento de Investigacao Criminal de Portimao da Polícia Judiciária e Directoria Nacional.

PROCESSO: NUIPC 201/07.0GALGS
NOME: MADELEINE BETH MCCANN.

OFICIO No. 013.079 and fax no. 399, both dated 08/05/2007, that say "to proceed to deliver the traces recovered from apartment no. 5-A of the tourist resort "The Ocean Club" in Praia da Luz in Lagos", which were accompanied by 7 (seven) sealed envelopes under a delivery note no. 156/2007-CR/L, as part of the investigation into the disappearance of the child MADELEINE BETH MCCANN.

OFICIO No. 13443 and fax no. 413 both dated 15/05/2007, that say to proceed to deliver the traces recovered from the residence at Rua do Ramalhete, Casa Liliana in Praia da Luz in Lagos (1 envelope), and from the Volkswagen with numberplate 44-77-KD (7 sealed envelopes), which were accompanied by delivery notes 170A/2007CR/L and 170B/2007-CR/L, respectively.

FAX No. 000415 dated 15/05/2007, that requests the INML to proceed with all necessary examinations on all the traces delivered to them as part of the abovementioned Inquiry.

OFICIO No. 13453 /I /2007 dated 15/05/2007, that says to proceed to deliver the bucal swabs recovered from Matthew David Oldfield, Jane Michelle Tanner, Gerald Patrick Mccann, Kate Marie Healy, with delivery note no. 170C/2007-CR/L and relevant official documents from the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria.

OFICIO No. 001803 dated 17/05/07, that says "to remit bucal swabs recovered from individuals, asking for comparisons of DNA profiles with those from biological traces recovered from various locations, as part of the present legal documents", accompanied by relevant official documents from the Departamento de lnvestigacao Criminal de Portimao da Polícia Judiciaria.

OFICIO No. 002026 dated 05/06/07, that says "to deliver two bucal swabs provided by Robert James Queriol Eveleigh Murat", with relevant official documents from the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria.
 
MATERIAL RECEIVED


There were received 258 traces and 12 reference samples - bucal swabs from 11 individuals.
 
DESCRIPTION OF MATERIAL RECEIVED


TRACES

Relating to Apartment no. 5-A in the tourist resort "The Ocean Club" Praia da Luz-Lagos:

- 32 hairs in envelope no. 1 recovered from the floor at the entrance to the children's bedroom.

- 28 hairs in envelope no. 2 recovered from the floor next to the bed from which the child disappeared.

- 4 hair in envelope no. 3 recovered from the top of the bed from which the child disappeared.

- 15 hairs in envelope no. 4 recovered from the floor next to the bed that was next to the window in the children's bedroom.

- 1 piece of cloth in envelope no. 5 recovered from the bedspread of the bed next to the window in the children's bedroom.
Fragment of cloth, mauve/violet in colour with square motifs, circular in form about 10cm in diameter. A small fluorescent spot is observed under a Crime-light.

- 31 hairs in envelope No. 6 recovered from the floor of the lounge.

- 58 hairs in envelope No. 7 recovered from the entrance hall at the front door of the apartment.

[The above were] Delivered by the Policia Judiciaria on 08/05/2007.

Relating to the Volkswagen 44-77-KD:

- 15 hairs in envelope No. 1 recovered from the front-right seat.

- 2 hairs in envelope No. 2 recovered from the front-left seat.

- 12 hairs in No. 3 recovered from the floor in front of the front seats.

- 7 hairs in envelope No. 4 recovered from the floor in front of the rear seat.

- 4 hairs in envelope No. 5 recovered from the rear seat.

- 5 hairs in envelope No. 6 recovered from the rear part of the vehicle.

- 4 hairs in envelope No. 7 recovered from the internal covering of the roof.

Relating to Residencia Casa Liliana - Praia da Luz-Lagos:

- 40 hairs in envelope No. 1 recovered from the bed in the bedroom of of the suspect.

[The above were] Delivered by the Policia Judiciaria on 15/05/2007.

REFERENCE SAMPLES

- 4 sealed bucal swabs from:
Matthew David Oldfield, Jane Michelle Tanner, Gerald Patrick McCann, Kate Marie Healey.

Delivered by the Policia Judiciaria on 15/05/2007.

- 6 sealed bucal swabs from:
Dianne Webster, Rachael Mariamma Jean Mampilly, Russell James O'Brien, Fiona Payne, David Anthony Payne, Silvia Maria Correia Batista.

Delivered by the Policia Judiciaria on 18/05/2007.

- 2 sealed bucal swabs from:
Robert James Queriol Eveleigh Murat.

Remitted by the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria and delivered on 06/06/2007.

METHODOLOGY

1-Morphology analysis of hairs

- Macro- and microscopic characterisation.

2-Nature of the sample

Acid Phosphatase Test to detect semen on the small spot on the cloth fragment in envelope no. 5 recovered from the bedspread of the bed next to the window of the children's bedroom.
[Ref: http://www.semenonpanties.com/laux.htm]

3- DNA extraction:

The following methods were used to extract DNA from the material received for analysis:

- Extraction with phenol-chloroform (organic extraction) [1,2]
[Ref: http://en.wikipedia.org/wiki/Phenol-chl ... extraction]
- Tissue and Hair Extraction kit -"DNA IQ(tm) System" (Promega)
- "QIAamp micro DNA kit" method (Qiagen)
- Chelex method [3]

[1] Huhne, J., Pfiffer, H., Waterkamp, K., Brinkmann, B. 1999. "Mitochondrial DNA in human hair shafts existence of intra-individual differences'". International Journal of Legal Medicine (1999), 112: 172-175.
[2] Maniatis T. Fritsch EF, Sambrook J. 1982. "Molecular Cloning - A Laboratory Manual". Cold Spring Harbor Laboratory.
[3] Walsh P.S., Metzer D.A., Higuchi (1991). Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.

4-Nuclear DNA study

After extraction the following genetic markers were studied by PCR:

- Autosomic STRs: D8S1179, D21S11, D7S820, CSFlPO, D3S1358, HUMTH01, D13S317, D16S539, D2S1338, D19S433, HUMVWA31/A, TPOX, D18S51, D5S818, HUMFIBRA/FGA and Amelogene, using the primers and conditions of amplification suggested in the kit AmpFISTR Identifiler (Applied Bioystems).

- Autosomic STRs: D3S1358, HUMTH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSFlPO, Penta D, HUMVWA31/A, D8S1179, TPOX, HUMFIBRA/FGA, and Amelogene using the primers and the conditions of amplification suggested in kit PowerPlex(tm)16 System (Promega).

5-Mitochondrial DNA study

The following methodology was used in the Mitochondrial DNA study:

PCR Amplification:

PCR Amplification of the human mtDNA regions HVI and HVII, using specified primers and amplification conditions in accordance with Vigilant et al., 1989 [4] e Wilson et al., 1995 [5], Alvarez-lglesias et al., [6].

[4] Vigilant L, Pennington R. Harpending 11, Kocher TD, Wilson AC. 1989. "Mitochondrial DNA Sequences in Single Hairs from a Southern African Population". Proc Natl Acad Sci, USA, 1989, Vol. 86: 9350 - 9354.
[5] Wilson MR, DiZinno JA, Polanskey D, Replogle J. Budowle B. 1995. "Validation of Mitochondrial DNA Sequencing for Forensic Casework Analysis". Int J Legal Med. 1995,108: 68 - 74.
[6] Alvarez-Iglesias V, Jaime JC, Carracedo A, Salas A.2007. "Coding region mitochondrial DNA SNPs:Targeting East Asian and Native American haplogroups" Forensic Sci. Int. Genetics, 2007, 1 :44-55.

Sequenciation:
Direct sequenciation of the HVI e HVll segments by capilar electrophoresis in "ABI PRISM 3100 ou 3130 Genetic Analyser" (Applied Biosystems) automated sequenciator with fluorescent detection using the following kits:

- "BigDye(R) Terminator vl.1 Cycle Sequencing" (Applied Biosystems)
- "BigDye(R) Terminator v1.3 Cycle Sequencing" (Applied Biosystems)
- "dRhodamine Terminator Cycle Sequencing Ready Reaction" (Applied Biosystems)

The programme "SeqScape(R) Software Version 2.0" (Applied Biosystems) was used for comparison of the sequences obtained from the analysed samples with the CRS (Cambridge Reference Sequence).

 
RESULTS


1- Morphology analysis of the hairs

All hairs received for analysis were subjected to macro- and microscopic characterisation, with the majority of those having the root being in a telogenic phase.
[Ref: https://www.promega.com/geneticidproc/e ... c/ab20.pdf]

Relating to Apartment no. 5-A

- Envelope No. 1 - "recovered from the floor at the entrance to the children's bedroom" 25 hairs with root; 4 hairs only stem; 3 hairs non-human.

- Envelope No. 2 - "recovered from the floor next to the bed from which the child disappeared" 3 hairs with root; 25 hairs only stem.

- Envelope No. 3 - "recovered from the top of the bed from which the child disappeared" 2 hairs with root; 2 hairs only stem.

- Envelope No. 4 - " recovered from the floor next to the bed that was next to the window in the children's bedroom" ll hairs with root; 4 hairs only stem.

- Envelope no. 6 - "recovered from the floor of the lounge" 26 hairs with root; 4 hairs only stem; l hair non-human.

- Envelope no. 7 - "recovered from the entrance hall at the front door of the apartment" 44 hairs with root; 8 hairs only stem; 6 hairs non-human.

Relating to the Volkswagen 44-77-KD

- Envelope no. 1 - "recovered from the front-right seat" 13 hairs with root; 1 hair root only; 1 hair non-human.

- Envelope no. 2 - "recovered from the front-left seat" 2 hairs with root.

- Envelope no. 3 - "recovered from the floor in front of the front seats" 9 hairs with root; 2 hairs only stem; 1 hair non-human.

- Envelope No. 4 - "recovered from the floor in front of the rear seat" 1 hair with root; 6 hairs only stem.

- Envelope No. 5 - "recovered from the rear seat" 4 hairs only stem.

- Envelope No. 6 - "recovered from the rear part of the vehicle" 1 hair with root; 4 hairs only stem.

- Envelope No. 7 - "recovered from the internal covering of the roof" 3 hairs with root; 1 hair only stem.

Relating to the Residencia Casa Liliana - Praia da Luz-Lagos:

- Envelope No. 1 - "recovered from the bed in the bedroom of of the suspect" 36 hairs with root; 4 hairs only stem.

2-Nature of the sample

Acid Phosphatase Test to detect
semen on the small spot on the cloth fragment in envelope no. 5 recovered from the bedspread of the bed next to the window of the children's bedroom: Weakly positive.

3 - Nuclear DNA study

Autosomic STRs:

- were obtained from: hair root CEnv 7-51 recovered in the apartment (entrance hall-Env.7), hair root PVT07 recovered in the vehicle (floor in front of rear seat Env.4), hair root C742-05 recovered in the vehicle (floor in front of the front seat-Env. 3) and the small spot on the piece of cloth (recovered from the bedspread of the bed next to the window in the children's bedroom Env.5).

- were obtained from swabs:
Matthew David Oldfield, Jane Michelle Tanner, Gerald Patrick McCann, Kate Marie Healey, Dianne Webster, Rachael Mariamma Jean Mampilly, Russell James O'Brien, Fiona Payne, David Anthony Payne, Silvia Maria Correia Batista e Robert James Queriol Eveleigh Murat.

4 - Mitochondrial DNA study

- Mitochondrial DNA was obtained from 194 vestiges - 1 spot and 193 hairs (roots and/or stems) of the 246 analysed. It was not possible to obtain results from 52 hairs, 31 of which were recovered in the apartment.

Mitochondrial DNA profiles of the vestiges

mtDNA    Number of                                    Comparison with
profile   Samples    Recovered from                   reference samples

B           3        3 entrance hall
B1          1        1 entrance hall
-------------------------------------------------------
                    13 lounge
                    15 entrance hall
C          53       13 entrance to bedroom            Kate Healy (Mother)
                     4 floor; bed next to window
                     1 bed of the child
                     7 floor; next to child's bed
-------------------------------------------------------
D           1        1 lounge
D1          1        1 entrance to bedroom
D1          1        1 floor; bed next to window
-------------------------------------------------------
F           3        1 lounge                               
                     2 entrance hall
-------------------------------------------------------
G           1        1 lounge                         Matthew Oldfield
I           1        1 entrance hall                  David Payne
-------------------------------------------------------
                     1 floor; bed next to window
J           3        1 entrance hall
                     1 floor; next to child's bed
-------------------------------------------------------
K          14        1 Residencia Liliana                     
                    13 vehicle(envlps 1,3,4,5 and 6)
-------------------------------------------------------
                     1 spot on bedspread
L           8        1 entrance to bedroom
                     2 entrance hall
                     4 floor; bed next to window
-------------------------------------------------------
M          48       13 vehicle (envlps 1,2,3 and 4)
                    35 Residencia Liliana             Robert Murat
-------------------------------------------------------
                     2 lounge
                    11 entrance hall
N          24        6 entrance to bedroom            Gerald Mccann
                     2 floor; bed next to window
                     3 floor; next to child's bed
-------------------------------------------------------
0           2        1 lounge
                     1 entrance hall                  Russell O'Brien
-------------------------------------------------------
Q           2        1 entrance hall
                     1 lounge                               
-------------------------------------------------------
Profiles different from each other and from those above:
           28       17 of which were found in the apartment.
 

* Bl and Dl have a different sequence from B and D.
 

CONCLUSIONS


1st- The macro- and microscopic analysis of 257 hairs revealed 245 human and 12 non-human.

2nd- With respect to autosomic STRs the genetic profiles of the following analysed samples matched with the reference sample indicated:

- hair root CEnv 7-51 recovered in the apartment (entrance hall-Env.7) matched with Gerald McCann, father of the victim.

- hair root PVT07 recovered in the vehicle (floor in front of rear seat Env.4) match with Robert James Queriol Eveleigh Murat.

3rd- With respect to autosomic STRs the female genetic profile identified from hair root C742-05 recovered in the vehicle (floor in front of the front seat- Env 3) did not match any profile from the reference samples.

4th- With respect to autosomic STRs the male genetic profile identified from the spot on the cloth fragment (bedspread of the bed next to window in the children's bedroom- Env 5) did not match any profile from the reference samples.

5th- In the samples from apartment 5-A, several mitochondrial DNA profiles were found:

- Profile identified by letter "C", present in 53 samples, was identical to that of Kate Healy, mother of the victim, meaning those samples were from her or from someone having the same maternal bloodline.

- Profile identified by letter "N", present in 24 samples, was identical to that of Gerald McCann, father of the victim, meaning those samples were from him or from someone having the same maternal bloodline.

- Profile identified by letter "G", present in 1 samples, was identical to that of Matthew David Oldfield, meaning that sample was from him or from someone having the same maternal bloodline.

- Profile identified by letter "I", present in 1 samples, was identical to that of David Anthony Payne, meaning that sample was from him or from someone having the same maternal bloodline.

- Profile identified by letter "O", present in 2 samples, was identical to that of Russell James O'Brien, meaning those samples were from him or from someone having the same maternal bloodline.

- Profile identified by letter "L", present in the spot on the bedspread of the bed next to the window and in seven hairs, meaning that all these samples came from the same person or from someone having the same maternal bloodline, did not match any of the reference samples.

- Profiles identified by letters "B", "D", "F", "J" and "Q" are different from the above, and from each other, and are distinct from reference samples.

6th- In the samples analysed from Residencia Liliana and the vehicle, several mitochondrial DNA profiles were found:

- Profile identified by letter "M", present in 48 samples (35 from Residencia Liliana and 13 from the vehicle), was identical to that of Robert James Queriol Eveleigh Murat meaning those samples were from him or from someone having the same maternal bloodline.

- Profile identified by letter "K", present in 14 samples (1 from Residencia Liliana and 13 from the vehicle), meaning that all these samples came from the same person or from someone having the same maternal bloodline, did not match any of the reference samples.

- One of the 13 samples from the vehicle in letter "K" - hair root C742-05 (floor in front of the front seat - Env 3) showed a female DNA genetic profile.

7th- The remaining 28 samples analysed, of which 17 were recovered from the apartment, showed mitochondrial DNA from different from each other, and distinct from those above.

8th- Of the 245 hairs analysed, no results were obtained from 52.

National Institute of Legal Medicine, 9 July 2007.
[Titles, names and signatures of signatories to the report]

2528 - Letter dated 28 August to the Police Science Laboratory asking them to preserve curtaining from apartment 5A

TRANSLATED BY INES

10 Processos Vol X Page 2528
10VOLUME_Xa_Page_2528


To: The Director of the Police Scientific Laboratory
Lisbon

Date: 28 August 2007

Subject:

The present inquiry deals with the investigation into the disappearance of the British girl, Madeleine McCann, a fact that occurred on 3rd May 2007 in Praia da Luz, Lagos.

During the course of the investigation diverse material/articles and objects susceptible to containing biological vestiges have been collected.

At the beginning of this month of August a curtain was collected from the living room of the apartment including a portion from the same pair of curtains, materials that were sent to England with other articles/objects for subjection to examination.

With the aim of preserving and maintaining the CHAIN and CUSTODY of evidence during the lack of conclusion from the examinations that are being carried out in England, the remaining inferior part of the curtain was collected.

Date and time of collection: 28th August 2007 06.35 hours
Place: Apartment 5 A, Ocean Club Resort, Praia da Luz

Accordingly we request that the piece collected and delivered to the Police Scientific Laboratory be PRESERVED for the eventual carrying out of complementary examinations.

With compliments

G. Amaral

The Coordinator of the Criminal Investigation

2529 to 2530 - Photographic Report undated but referenced as 28 August 2007 on page 2528 above
10 Processos Vol X Pages 2529 to 2530
10VOLUME_Xa_Page_2529 10VOLUME_Xa_Page_2530

Photographic Report of the Technical Police Nucleus

Place: Ocean Club

Photos: 2

Assistant Specialist: J. Barreiras


Photos:

2969 to 2970 - Fax to Police Scientific Lab 26 September 2007 requesting forensic/DNA examinations
2971
- confirmation of above fax

TRANSLATED BY INES

11 Processos Vol XI Pages 2969 - 2970
 11_VOLUME_XIa_Page_2969
 
11_VOLUME_XIa_Page_2970
 
11_VOLUME_XIa_Page_2971
 

Fax

Ref 00 731

Confidential

To: The Director of the Scientific Police Laboratory

From PJ, DIC Portimao

Date: 26-09-2007

Subject: Request for Examinations

Ref: NUIPC 201.07 Galgs


As it necessary for the investigation of the crime mentioned above and is of a VERY URGENT nature, I request you to carry out the examinations of the material already sent to this laboratory as quickly as possible, namely:

1. Swabs with vestiges of human blood collected by officers from the local crime scene (examination no. 200707356 - CR/L) on 6th May 2007 from the wall of Quinta dos Figos, P da L. Material preserved in this laboratory (examination no. 200707143-BG). For the determination of DNA and comparison with the profiles that were obtained in the examinations carried out by the INML within the scope of the process 2007000226/LX-BC.

2. Curtain collected from apartment 5 A of the OC resort, P da L on 28th August 2007 and delivered to this laboratory on the same day where it is preserved (examination no. 200712288- BG. For the search of vestiges namely blood, semen or hair. According to the results of the examinations further tests will be considered.

With compliments,

The Coordinator of the Criminal Investigation

Goncalo Amaral


PT text:
 

2972 - Fax 26 September 2007 to Director of National Institute of Legal Medicine asking for DNA profiles to be sent to the LPC
2973 - Confirmation of above fax
11 Processos Vol XI Page 2972
11_VOLUME_XIa_Page_2972

 11_VOLUME_XIa_Page_2973

 

Fax

Ref 000730

Confidential

To: The Director of the INML

From: PJ DIC Portimao

Date: 26-09-2007

Ref : Process 2007000226/LX-BC

NUIPC 201-07 Galgs

As it is necessary for the investigation of the crime mentioned above, namely with the aim of making comparisons with the material that is being examined by the Scientific Police Laboratory of the PJ, I hereby request that you send the DNA profiles from your examination no. 2007000226/LX-BC obtained to the Laboratory.

With compliments

The Coordinator of the Criminal Investigation

Goncalo Amaral

2977 - Fax 1 October 2007 from INML asking which profiles were to be sent to LPC
11 Processos Vol XI Page 2977
 11_VOLUME_XIa_Page_2977
 

Fax
To: PJ DIC Portimao
From: INML

Date: 1st October 2007 Ref: 2007000226LX-BG

Subject: DNA Profile

Ref: Your fax 730 of 26-09-2007

I ask you to indicate whether you are referring to the mitochondrial DNA profiles or the results of the DNA profiles obtained in autosommic STRs with regard to the samples in the reference.
With compliments

The Director of the Forensic Genetic and Biological Service
Dra Espinheira

2978 - Fax 2 October 2007 responding to INML query (page 2977): full DNA profiles from samples to be sent to LPC
11 Processos Vol XI Page 2978
 11_VOLUME_XIa_Page_2978
 


Fax No. 741

Confidential
Date: 3rd October 2007

To: The Director of the INML
From: PJ, DIC Portimao

Ref: Your fax

In response to your fax no. 282 412763 of 1st October 2007 I am pleased to inform you that I am requesting that you send the Scientific Police Laboratory the results of the DNA profiles obtained from autosommic STRs relating to all the samples examined by this Institute in the scope of your examination no. 2007-000226 LX-BG.
With compliments

The Coordinator of the Criminal Investigation
Goncalo Amaral
 

2982 - Information file note 1 October 2007 stating that the PJ has had to request a copy of Madeleine's DNA from Scotland Yard for use in DNA comparisons to be made in the Portuguese forensic laboratories.
11 Processos Vol XI Page 2982 
also Outros Apensos I, Vol I Page 269
11_VOLUME_XIa_Page_2982

Information

With the aim of providing the Scientific Police Laboratory with the DNA profile of Madeleine McCann so that they are able to make comparisons with the DNA profiles that have been identified in the examinations requested, on this date and within the framework of the cooperation between the PJ and the UK authorities, I made a telephone call to Jose de Freitas, an officer with New Scotland Yard and asked him to send me by email the girl's profile that was determined by examinations carried out in a UK laboratory, referred to previously in a letter that is part of this process.

In response to my request, Jose de Freitas sent me an email, a copy of which I immediately sent to the director of the SPL, Dr Saudade Nunes.


Portimao, 1st October 2007

Inspector Paolo Ferreira

 

Followed by email (with an attachment)

 

From Jose de Freitas

New Scotland Yard to Inspector Ferreira

 

 

Inspector Ferreira,

 

 

DNA profile of Madeleine McCann that was collected in her parents house in England.

 

 

Regards

 

 

Jose de Freitas

New Scotland Yard

2983 to 2994 - Email 2 October 2007 from Jose Freitas, New Scotland Yard re: Madeleine's DNA profile obtained from the FSS
11 Processos Vol XI Page 2983 to 2994
Page 2983

Email

From: Jose de Freitas
To: Paolo Ferreira

Date: 2 October 2007

Subject: DNA profile Madeleine McCann

Inspector Paolo Ferreira

DNA profile of Madeleine McCann, which was collected from her parents home in England.
Compliments

Jose de Freitas
New Scotland Yard.
 
11_VOLUME_XIa_Page_2983 11_VOLUME_XIa_Page_2984 11_VOLUME_XIa_Page_2985
11_VOLUME_XIa_Page_2986 11_VOLUME_XIa_Page_2987 11_VOLUME_XIa_Page_2988
11_VOLUME_XIa_Page_2989 11_VOLUME_XIa_Page_2990 11_VOLUME_XIa_Page_2991
11_VOLUME_XIa_Page_2992 11_VOLUME_XIa_Page_2993 11_VOLUME_XIa_Page_2994
3207 - Delivery note 304-B sent to LPC 14 August 2007 with fibres (item 26) for analysis
TRANSLATION BY INES
12 Processos Vol XII Page 3207
12_VOLUME_XIIa_Page_3207

Delivery Guide no. 304B/2007 CRL

Delivery Guide of Vestiges Collected from the Crime Scene

Date of Delivery : 14 August 2007

Destination: LPC Physical Area

Forensic examination no.: 200711732 CRL

NUIPC: 201/07 GALGS

Date and Time of Collection: 020630 August 2007

Place of Collection: Wall of apartment 5 A, OC, P da L.

Scope: Disappearance of English girl

Requested by: PJ DIC Portimao
Vestiges Delivered:

No. Description

26 Crystal Tabs containing eventual fibres

Observation: This material is for preservation and to be kept for possible laboratory examination.

Lisbon 14th August 2007

Delivered Received

Signature Signature

 

3208 - Delivery note 304-A sent to LPC 14 August 2007 with hair (item 25) for analysis
12 Processos Vol XII Page 3208
12_VOLUME_XIIa_Page_3208

Delivery Guide no. 304 A 2007 CRL

Delivery Guide of Vestiges Collected from the Crime Scene

Date of Delivery : 14th August 2007

Destination: LPC Biology Area

Forensic Examination no. 200711732 CR/L

NUIPC: 201/07. OGALGS

Date and Time of Collection: 020630 August 07

Place of Collection. Apartment 5 A, OC P da L.

Scope: Disappearance of English girl

Requested by: PJ DIC Portimao

Vestiges Delivered:

No. Description

25 Diverse hairs

Observation: This material is for preservation and to kept for possible laboratory examination.

Lisbon. 14th August 2007


Delivered Received

Signature Signature

3224 to 3225  Delivery note 286-A listing 33 swabs of stains/evidence collected from 5A (English) - signed for by FSS on 7 August 2007

TRANSLATED BY INES

12 Processos Vol XII Page 3224 to 3225
12_VOLUME_XIIa_Page_3224
12_VOLUME_XIIa_Page_3225
Processos Vol XII
Pages 3224 - 3225 (in English)

Delivery Guide 286 A - 2007

Delivery Guide of Recovered Evidences at the Crime Scene

In order to carry out the determined by the 4th Brigade of the PJ Criminal Investigation Department, concerning to process number 201/07 Galgs, between 15.00 of August 4th 2007 and 06.30 of August 5th 2007, were recovered the following evidences in the living room of the apartment 5 A. Ocean Club villas, Praia da Luz, Lagos, Portugal, where is possible may had occurred a crime of homicide, by the Crime Scene Investigators Fernando Jose da Silva Viegas and Bruno Jorge Possidonio Mendes Atunes:

1A. Stain on the floor recovered with a dry swab.
1B. Stain on the floor recovered with swab, with distilled water.
2A. Stain on the floor recovered with a dry swab.
2B. Stain on the floor recovered with a swab, with distilled water.
3A. Stain on the floor recovered with a dry swab.
3B. Stain on the floor recovered with a swab, with distilled water.
4A. Stain on the floor recovered with a dry swab.
4B. Stain on the floor recovered with a swab, with distilled water.
5A. Stain on the wall with a dry swab.
5B. Stain on the wall recovered with a swab with distilled water.
6A. Stain on the floor recovered with a dry swab.
6B. Stain on the wall recovered with a swab with distilled water.
7A. Stain on the floor recovered with a dry swab.
7B. Stain on the wall recovered with a swab with distilled water.
8A. Stain on the floor recovered with a dry swab.
8B. Stain on the wall recovered with a swab with distilled water.
9A. Stain on the floor recovered with a dry swab.
9B. Stain on the wall recovered with a swab with distilled water.
10A. Stain on the floor recovered with a dry swab.
10B. Stain on the wall recovered with a swab with distilled water.
11A. Stain on the floor recovered with a dry swab.
11B. Stain on the wall recovered with a swab with distilled water.
12A. Stain on the floor recovered with a dry swab.
12B. Stain on the wall recovered with a swab with distilled water.
13A. Stain on the floor recovered with a dry swab.
13B. Stain on the wall recovered with a swab with distilled water.
14A. Stain on the back of a sofa recovered with a dry swab.
14B. Stain on the back of a sofa recovered with a swab with distilled water.
15A. Stain on the back of a sofa recovered with a dry swab.
15B Stain on the back of the sofa recovered with swab with distilled water
16. Blue curtain
16. Blue curtain.
16B. White curtain behind blue curtain and armband.

All this evidences were delivered in the Forensic Science Laboratory - Birmingham Laboratory, Priory House, Gooch Street North, Birmingham, B56QQ on August 7th, 2007.

Birmingham, August 7th 2007

Who delivers Who received

Signed Signed
3242 to 3244 - Delivery note 286-C listing 34 items of evidence found in Renault Scenic - signed for by FSS on 7 August 2007 (English)
12 Processos Vol XII Pages 3242
Delivery note 286-C listing 34 items of evidence found in Renault Scenic - signed for by FSS on 7th August 2007 (English)
12_VOLUME_XIIa_Page_3242
12_VOLUME_XIIa_Page_3243
3252 to 3253 Letter from the National Institute of Legal Medicine re: 3 DNA non-identified profiles

 TRANSLATED BY ALBYM

12-Processos Vol XII Pages 3252 to 3253
12_VOLUME_XIIa_Page_3252
12_VOLUME_XIIa_Page_3253
From Goncalo Amaral

To: INML

Date: 4th September 2007

The present request deals with the investigation into the disappearance of Madeleine McCann on 3rd May 2007.

Within the scope of this request we have asked the INML to carry out various analyses as your letter 2007000226 LX / BC reports.


The results were delivered to us in a letter dated 9th July 2007 ref 13.079, with a cover letter dated 11th July 2007.

Upon reading the report we observed that several DNA profiles are defined but which have not been identified.


The apartment from which the samples were collected for analysis is occupied by families from the UK.

The UK police and legal authorities have informed us about the existence of a database for the register of DNA profiles.

As the objective is to proceed to identify the profiles defined in your analyses we request that you send us the list of unidentified profiles.
This request has the objective of seeking their eventual identification through the UK legal authorities.

Goncalo Amaral
3265 - Fax 4 October 2007 re: delivery of DNA profiles
12 Processos Vol XII Page 3265
12_VOLUME_XIIa_Page_3265

Fax

From INML

To: Policia Judiciaria, Portimao DIC

From: The Forensic Genetics and Biology Service

Attention of Inspector Paolo Ferreira

Date: 4th October 2007

Subject: DNA Profile


Your reference number: NUIPC 201-07.OGALGS - Fax no. 741 of 03-10-2007

Notes/ Comments:

On 04-10-2007 the requested DNA profiles were sent to the Scientific Police Laboratory of the Policia Judiciaria.

With compliments


The Director of the Forensic Genetics and Biology Service

Signed

3476 to 3478  Forensic report 15 November 2007 on four swabs per request of 8 Nov

TRANSLATED BY INES

13 Processos Vol XIII Pages 3476-3478
13_VOLUME_XIIIa_Page_3476
13_VOLUME_XIIIa_Page_3477
13_VOLUME_XIIIa_Page_3478
(Note: these examinations refer to the material collected at the Burgau apartment)

Forensic Examination Report

number 200707143 - BG

Requested by: Portimao DIC

Process Number: 201/07 OGALGS
731 of 28th September 2007
618 and 619 of 2nd November 2007
034308 of 4th October 2007
Email message with annex sent on 8th November 2007

Delivery Guides: 189/2007- CRL of 24th May 2007
196/2007 of 29th May 2007

Examination initiated on: 8th November 2007.


Material for examination

1. One swab referenced as Vestige number 1 from the floor next to the wall.
2. One swab referenced as Vestige number 2 from the top of the wall.
3. One swab referenced as Vestige number 3 from the floor next to the wall.
4. One swab references as Vestige number 1A from the door of the kitchen cupboard.

Requests

The examination of the haematic material received for examination is requested with respect to the DNA analysis and a comparative study with the profiles sent via email on 8th November and in letter number 034308 of 4th October 2007

Observations and Tests Carried Out

The swabs described in lines 1, 2 and 3 were stained with brownish red.

The swab described in line 4 was very slightly stained in a yellow tone.

Preliminary Tests

Search for peroxidase existing in the blood using the Kastle Mayer test.

DNA Analysis

Methods used for DNA extraction.

Extraction of DNA from the blood samples using the organic method (Phenol/Chloroform).
- 1 to 4 swabs

Amplification of DNA using the P.C.R. technique.

The amplification of the DNA was carried out using the P.C.R technique (Polymerase Chain Reaction) and was analysed according to the following:

- D3S1358, VWA, D16S539, D2S1338, Amelogenina (sex), D8S1179, D21S11, D18S51,
- D19S433, TH01 and FGA using primers marked with fluorochromes and amplification conditions in accordance with the protocol described in AmpFISTR SGM Plus PCR amplification Kit.

Detection and Analysis of Amplified DNA

The detection and analysis of the amplified DNA was carried out in the automatic sequencer ABI Prism Genetic Analyser.

Conclusion

- In the swabs described in lines 1, 2 and 3, blood vestiges were found.
- In the swab described in line 4 very slight traces of blood were found.
- In the DNA analysis of the haematic vestiges detected in the swabs described in lines 1 and 2 a profile was obtained belonging to an individual of the male sex.
- In the DNA analysis of the swab described in line 3 only some results were obtained from the loci that were studied. According to the results obtained a mixture of biological traces belonging to more than one individual were found, it only being possible to indicate the profile of the main contributor who presented an identity of polymorphisms for a set of loci studied with the profile described in the previous line.
- There is no identity of polymorphisms of the haematic vestiges detected in the swabs described in lines 1, 2 and 3 with the profiles sent by email on 8th November 2007 and in the letter number 034308 of the 4th October 2007.
- In the DNA analysis of the haematic vestiges detected in the swab described in line 4 results were only obtained from some of the loci studied, corresponding to the profile of an individual of the female sex. According to the results obtained, there is no polymorphism identity with the profiles sent by email on 8th November 2007 and in the letter number 034308 of the 4th October 2007.

Return of material: return the remains of the material received for examination.

15th November 2007


Signed

Technician Checked by
Superior Specialist Superior Specialist
3480 to 3481 - Forensic report 14 November 2007 on curtaining per request of 8 Nov
Processos Vol XIII Pages 3480 to 3481
13_VOLUME_XIIIa_Page_3480
13_VOLUME_XIIIa_Page_3481



Policia Judiciaria


Report of Forensic Examination
No. 200712288-BG


Requesting Party: Portimao DIC - PJ


Process No.: 201/07.OGALGS
2936 of 28 August 2007
731 of 28 September 2007
034308 of 4 October 2007
619 of 2 November 2007
Email message with annex sent on 8 November 2007.


Examination initiated on 8 November 2007


Material for Examination

1. Piece of cloth in a white tone, referred to in the Report on Collection of Vestiges No. 254-07 as 'taken from the large window on the eastern side' (Photo on page two).

Request.

It is requested that a search be made of the biological vestiges on the material received for examination, respective DNA analysis and comparative study with the profiles sent, via email on 8th November 2007 and by means of correspondence no. 034308 of 4th October 2007.

Observations and Tests Carried Out.

Preliminary Tests


- Search for peroxide existing in blood using the Kastle-Mayer test.
- Observation using the SL-450 Lamp (AG Projection).
- Search for phosphate existing in semen using the Acid-Phosphate test.

Conclusion.

On the piece of cloth described in line 1 no vestiges of blood or semen were detected.
On the piece of cloth described in line 1 no hairs were detected.


Return of material: Return the material received for examination.

Lisbon, 14th November 2007

Signed

Scientific Police Laboratory.
Biology Area
Lisbon

3483 to 3487 Forensic report 23 November 2007 on Eeyore pyjamas (+ photographs)
13 Processos Vol XIII Pages 3483 to 3487
13_VOLUME_XIIIa_Page_3483
 
13_VOLUME_XIIIa_Page_3484
 
13_VOLUME_XIIIa_Page_3485
 
13_VOLUME_XIIIa_Page_3486
 
13_VOLUME_XIIIa_Page_3487
 

Forensic report No. 200711800-FI

Requested by:: Portimao DIC

NUIPC 201-07 Galgs

Correspondence number: 3609 of 31st October 2007

Examination initiated : October 2007


Material for Examination

I. Six collection kits of woven fibres which present the following references relating to the area of vestige collection:

1. 'Inferior part of wall' and 'Pip'.
2. 'Superior part of wall' and 'PSP'
3. 'Middle part of wall' and 'PMP'
4. 'Middle part of wall' and 'PMP'
5. 'Superior part of wall' and 'PSP'
6. 'Inferior part of wall' and 'Pip'.

Each kit comes inside an orange coloured envelope, each of these envelopes are inside a paper envelope with the following references, amongst others 'Vestige No. 26, Apartment 5 A, OC resort, P da L. Garden wall of the apartment, 2007/08/02'.


II - A pyjama camisole, pink in colour with motifs stamped and sewn on to the front (the latter of material, buttons and messages) (see photographs 1 and 2). Three labels can be seen, one of them has the following references, amongst others: 'Top 100% COTTON EXCLUSIVE OF TRIMMINGS'.

III - White coloured pyjama bottoms with stamped designs in pink shades (different tones), green and yellow and with a trim at the bottom of each leg (see photo 3 and 4). Three labels can be observed, one of them with the following reference, amongst others 'Bottom 100% COTTON EXCLUSIVE OF TRIMMINGS'.



REQUEST

We request the examination of the samples collected from the garden wall with the aim of carrying out the 'search and identification of eventual fibres and their comparison with the two parts of the pyjamas similar to those the girl was wearing when she disappeared'.

OBSERVATIONS AND TESTS CARRIED OUT

With the objective of identifying the fibres from the searches for samples from the garden wall, it was proceeded to identify the prime material of the pyjamas, from which we had previously collected reference samples from both the top and the bottom. Identification was done using an optical microscope, the following results were obtained:

Pyjama camisole:

The front was made up of white base thread and external pink thread.

- Prime material of the white thread: cotton and synthetic fibre (very pigmented).
- Prime material of the pink thread: cotton (the colouring was not visible by microscope).

The back of the top consisted of pink coloured thread

- Prime material of the pink thread: cotton (the colouring was not visible by microscope).


The Pyjama bottoms:

The material of the pyjama bottoms consisted of white coloured thread.

- Prime material of the thread: cotton

Observations using a stereoscopical magnifier and an optical microscope

With the objective of comparing eventual textile fibres collected from the wall with the fibres from the pyjamas, a search was made of the textile fibres (synthetic white fibres and white and pink cotton fibres) from the samples collected for examination. The search was carried out using a stereoscopical magnifier and an optical microscope, pink and white cotton fibres having been selected as well as white synthetic fibres for subsequent comparison.


Comparison Microscope

The fibres selected were compared to the fibres from the pyjamas using an optical comparison microscope. The following results were obtained:

- No synthetic fibres were detected that were compatible with the pyjama fibres.

- In relation to the cotton fibres, although numerous white cotton fibres were detected in all the samples, in none of them were vestiges detected that enabled discriminatory comparative analyses (such as for example, vestiges of stamping).

CONCLUSION

The analysis of the samples collected from the garden wall of the apartment of the OC yielded the following results:

- No synthetic fibres compatible with the fibres received for comparison from the pyjamas were found.
- Numerous white cotton fibres were found. However, this result should not be considered to be significant, given that according to studies of distribution and persistence of textile fibres it should always be possible to find fibres of different types and colours on any surface, white cotton fibres being very common and without discriminatory parameters.
- In addition, the interval of time that has passed between the events being investigated and the collection of samples (out in the open air) means the existence of fibres from the date of the events is of very low probability.

Note: The material remains archived at the SPL.

Lisbon, 23rd November 2007

Signed

Superior Specialist Machado Area Chief Monteiro

3591 Translated cover letter re: Eeyore pyjamas for testing
13 -Processos Vol XIII Page 3591
Translated cover letter re: Eeyore pyjamas for testing (no date)
13_VOLUME_XIIIa_Page_3591
3950 to 3953 Letters re: analysis of fibres/hair samples

TRANSLATED BY ALBYM

13-Processo Vol XIII Page 3950
13_VOLUME_XIIIa_Page_3950
13_VOLUME_XIIIa_Page_3951
13_VOLUME_XIIIa_Page_3952
13_VOLUME_XIIIa_Page_3953

To The Director of the Police Scientific Laboratory

Date: 31 -10- 2007

Subject: Request for forensic analysis

Within the context of the current investigation into the disappearance of Madeleine McCann on 3rd May 2007.

As it is necessary for the abovementioned investigation and is of a VERY URGENT nature, I request that you proceed to analyse, as quickly as possible, the appropriate analyses of the six Crystal Tabs containing eventual fibres collected by Local Crime Scene Officers (examination 200711732-CR/L and delivery guide 304B/2007 - CR/L) on 1st August 2007 from a wall of apartment 5 A of the OC, these are preserved in the physical area of this laboratory (2007 11800-F1).

It is a case of searching and identifying the eventual fibres and comparing them to the fibres from a pair of pyjamas similar to those the girl was wearing when she disappeared.


For this purpose I am sending the pyjamas and request that the examination be carried out as quickly as possible and that subsequently the pyjamas be returned to this department in order to send them to the forensic laboratory in the UK.

Paolo Rebelo

3954 to 3959 Letters of delivery to the National Institute for Legal Medicine with samples 8 November 2007
13 Processo 13 Pages 3954 to 3959
13_VOLUME_XIIIa_Page_3954 13_VOLUME_XIIIa_Page_3955 13_VOLUME_XIIIa_Page_3956
13_VOLUME_XIIIa_Page_3957 13_VOLUME_XIIIa_Page_3958 13_VOLUME_XIIIa_Page_3959
3960 to 3961 Fax re: request for exams
3962 Confirmation above fax sent
13-Processos Vol XIII Pages 3960-3961
13_VOLUME_XIIIa_Page_3960
13_VOLUME_XIIIa_Page_3961
13_VOLUME_XIIIa_Page_3962

Date: 31 October 2007
To: The Director of the Police Scientific Laboratory

From: Portimao PJ

Subject: Request for Examinations


Having noted the existence of two errors in the content of Fax no. 000731 of 26-09-2007, I ask you to take into consideration the following modifications:


In point one where it says examination no. 200707356-CR/L it should say examination no. 200707116-CR/L and in delivery note no. 189/2007/CR/L it should read 'Praia' instead of 'Paria'.


In point two, where it says 'Examine the samples, namely blood, semen or hair. The results of these examinations will be compared with other forensics'. It should say 'Examine the biological samples and their respective DNA profiles'.


We also request that the DNA profiles identified during the forensic tests requested by fax no. 000731 of 26-09-2007 are compared with the DNA profile of Madeleine McCann and the DNA profiles sent to the LPC by the Delegation of the INML within the process 2007/000226/LX-DC in letter 034308 of 4th October 2007.
With compliments
Paolo Rebelo

3963 Fax re: request for expert analysis
13-Processos Vol XIII Page 3963
13_VOLUME_XIIIa_Page_3963

To the Director of the Police Scientific Laboratory

From: Paolo Rebelo

Date: 31st October 2007

Subject: Request for Forensic Analysis


As it is necessary for the investigation of the abovementioned crime and being of a VERY URGENT nature, I request that the appropriate analyses be carried out as quickly as possible on the human blood samples collected by officers from the Local Crime Scene Unit (examination 200707356-CR IL of delivery guide 196/2007 - CRL on 5th May 2007 in apartment c, 2nd floor of aparthotel Sol e Mar in Burgau. These samples are in the Biology Area (200707143-B6).
We require the determination of the DNA profile and a comparison with the DNA profile of Madeleine McCann and the DNA profiles sent to the Police Scientific Lab from the INML within this process no. 2007/000226/LX - BC by means of communication no. 034308 of 4th October 2007.

Paolo Rebelo

3964 - Confirmation above fax sent
3965
- Fax re: request for delivery of vestiges 2 November 2007
3966 - Confirmation above fax sent
3967 - Fax re: request for delivery of vestiges 2 November 2007
3968 - Confirmation above fax sent
13-Processos Vol XIII Page 3964 to 3968
13_VOLUME_XIIIa_Page_3964 13_VOLUME_XIIIa_Page_3965 13_VOLUME_XIIIa_Page_3966
13_VOLUME_XIIIa_Page_3967 13_VOLUME_XIIIa_Page_3968  
 
3969 Fax re: request for delivery of vestiges
3970
- Confirmation above fax sent
13- Processo Vol XIII Page 3969 to 3970
13_VOLUME_XIIIa_Page_3969 13_VOLUME_XIIIa_Page_3970
Fax to the Director of the Police Scientific Laboratory

From: The PJ

Date: 31-10-2007

Subject: Request for the delivery of samples to the INML


As it is necessary for the abovementioned investigation, and being of a VERY URGENT nature we request that you deliver, as quickly as possible, the different hairs collected from the wall of apartment 5 A by Local Crime Scene Officers to the INML in order that the appropriate analyses be carried out (examination 200711732-CRIL delivery guide 304 A/2007) dated 1st August 2007. The samples are in the biology area of this laboratory (2007711865- BG).

Paolo Rebelo
3971 Fax re: request for delivery of vestiges
13- Processo - Vol XIII Page 3971
13_VOLUME_XIIIa_Page_3971

Fax to the Director of the Police Scientific Laboratory

From: The PJ

Date: 31-10-2007

Subject: Request for the delivery of samples to the INML

As it is necessary for the investigation of the abovementioned crime and being of a VERY URGENT nature I request delivery to the INML, as soon as possible, of the various hair samples collected by the Local Crime Scene officers from apartment C, 2 Aparthotel Sol e Mar in Burgau (examination 200707356-CR/L, delivery guide no. 196/2007 CR-L). These samples were collected on 5th May 2007 and are preserved in the Biology Laboratory of the Police Scientific Lab (examination 200707143-BG).
Paolo Rebelo

3972 - Confirmation above fax sent
3973 - Fax re: request for delivery of vestiges 2 November 2007
3974 - Confirmation above fax sent
3975 - Fax re: request for delivery of vestiges 2 November 2007
13-Processos Vol XIII Page 3972 to 3975
13_VOLUME_XIIIa_Page_3972 13_VOLUME_XIIIa_Page_3973 13_VOLUME_XIIIa_Page_3974 13_VOLUME_XIIIa_Page_3975
3976 Confirmation above fax sent
3977  Documentation re: cancellation of exam 15 November 2007
13-Processos Vol XIII Page 3976
13_VOLUME_XIIIa_Page_3976
13_VOLUME_XIIIa_Page_3977

Fax

To The Coordinator of the Criminal Investigation


Date: 2007/11/15


Subject: Examination no. 200708357-BG

In relation to the examination requested within the context of the investigation referred to above, I hereby inform you that this was CANCELLED, the material received by the Police Scientific Laboratory being returned as requested, to the Genetics and Biological Forensic Services of the INML in Lisbon.


With compliments.
Director of the LPC

3978  Documentation re: cancellation of exam
13-Processos Vol XIII Page 3978
13_VOLUME_XIIIa_Page_3978

Fax

To The Coordinator of the Criminal Investigation

Date: 2007/11/15

Subject: Examination no. 200708359-BG

In relation to the examination requested within the context of the investigation referred to above, I hereby inform you that this was CANCELLED, the material received by the Police Scientific Laboratory being returned as requested, to the Genetics and Biological Forensic Services of the INML in Lisbon.

With compliments.
Director of the LPC

(Note: these would appear to be a test on samples from the Renault Kangoo)

3979 Documentation re: cancellation of exam
13-Processos Vol XIII Page 3979
13_VOLUME_XIIIa_Page_3979

Fax
To The Coordinator of the Criminal Investigation

Date: 2007/11/15

Subject: Examination no. 200708360-BG

In relation to the examination requested within the context of the investigation referred to above, I hereby inform you that this was CANCELLED, the material received by the Police Scientific Laboratory being returned as requested, to the Genetics and Biological Forensic Services of the INML in Lisbon.

With compliments.

Director of the LPC

3980 Documentation re: cancellation of exam
13-Processos Vol XIII Page 3980
13_VOLUME_XIIIa_Page_3980

Fax
To The Coordinator of the Criminal Investigation

Date: 2007/11/15

Subject: Examination no. 200711865-BG

In relation to the examination requested within the context of the investigation referred to above, I hereby inform you that this was CANCELLED, the material received by the Police Scientific Laboratory being returned as requested, to the Genetics and Biological Forensic Services of the INML in Lisbon.

With compliments.

Director of the LPC

3891 Returned fibres found in Renault Scenic from the FSS

TRANSLATION BY INES

15 Processos Vol XV Page 3891
15VOLUMEXVa_Page_3891
To: The Director
INML
Lisbon

Ref: NUIPC 201/070 GALGS
4th Brigade
Inspector Joao Carlos


Date: 12th December 2007

Subject: Request for Forensic Examination


I am delivering the following to you, a component covered with fibre and a plastic component, both extracted from the luggage boot of the Renault Scenic vehicle used by the parents of Madeleine McCann, material which has already been examined by the forensic lab of the FSS in Birmingham, UK and which was returned to us, requesting your collaboration in the sense of proceeding to carry out the necessary examinations of the material in question, using the appropriate methodology, bearing in mind the detection of eventual biological vestiges that might not have been detected in the first examination that they were subject to.

With compliments

The Coordinator of the Criminal Investigation
Paulo Rebelo


(Handritten note of reception of two pieces, by Teresa 14-12-2007)

 
3892  Request for forensic exam of spot found on mattress in 5A
15 Processos Vol XV Page 3892
15VOLUMEXVa_Page_3892
To:

The Director of the INML
Lisbon Delegation



Date : 12th December 2007

Ref: 201/07 GALGS
4th Brigade
Officer Joao Carlos


Subject: Request for Forensic Examinations

With reference to the report dated 09-07-2007 (process 2007000268 P/B, process 2007000244 CR - B and process 20070026 LX BC) we request you to carry out the examinations you consider appropriate, bearing in mind the nature of the stain (found on the mattress of one of the beds in apartment 5 A of the OC, P da L) which enabled the identification of the profile identified by the letter L.

With compliments

Coordinator of the Criminal Investigation

Paolo Rebelo
3940 to 3941 - University of Texas Missing Persons DNA Database Report
3945 to 3947 - Portuguese translation of pages 3940 to 3941
15 Processos Vol XV Pages 3940  to 3941
15VOLUMEXVa_Page_3940
 
15VOLUMEXVa_Page_3941
 
15VOLUMEXVa_Page_3945
 
15VOLUMEXVa_Page_3946
 
15VOLUMEXVa_Page_3947
 

(In English)
From the University of North Texas
Missing Persons DNA Database Report

December 6, 2007

Accession Number1 : 07/5949
Accession Number 2: 07-6008

Submitting Agency 1
Galveston County Medical Examiner

Submitting Agency 2: FBI

Items received


Received via Mark Ingraham on November 8, 2007:

07-5949.1 Unidentified Remains Sample, Bone.

Received via email from SA Gay on November 20, 2007:

07-6008-1 Genetic data, STR Profile of Madeleine McCann.

Results

Human DNA extracted from item 07-5949.1 was analysed for thirteen genetic loci using the Applied Biosystems AmpFISTR Profiler Plus ID and Cofiler systems. Genetic data for item 07-6008.1 was sent by the FBI.

The genetic data obtained from item 07-5949 was uploaded into the Unidentified Human /Remains index of the Combined DNA Index System (CODIS). At the request of the submitting agency, the data obtained from the unidentified remains 07-5949.1 was compared to the data sent associated with item 07.6008.1.

The nuclear genetic data associated with items 07-5949.1 and 07-6008.1 do not match. Therefore Madeleine McCann is excluded as a potential contributor of the unidentified remains associated with item 07-5949.1.

A portion of the remitted remains will be maintained by the UNT Center for Human Identification Laboratory.

In the event that you have any questions or if we can be of further assistance, please feel free to contact the laboratory at*****
The above is the opinion of the undersigned, and this report shall not be reproduced without verbal or written permission.
Signed
Forensic analysts.

3942 - FBI Analysis of Madeleine and Baby Grace DNA report -  no match
3943 to 3944 - Portuguese translation of page 3942
15 Processos Vol XV Page3942 to 3944
15VOLUMEXVa_Page_3942
 
15VOLUMEXVa_Page_3943
 
15VOLUMEXVa_Page_3944
 

(In English)

US Dept of Justice

Federal Bureau of Investigation

Office of the Legal Attache, Madrid, Spain
21st January 2008

To: Portuguese Judicial Police

Subject: DNA Comparison for victim Madeleine McCann

On 05 November 2007, the Portuguese Judicial Police (PJ) requested the FBI conduct a DNA comparison from the known DNA of victim Madeleine McCann to that of a body of a minor female (since identified as deceased minor victim Riley Ana Sawyers) that washed ashore near Galveston, Texas, USA on or about 04 November 2007.

On 05 November 2007, the FBI's legal attache office in Madrid, Spain forwarded the known DNA sample provided by the PJ to the FBI Field Office in Houston, Texas, USA. Subsequently, the known DNA of Madeleine McCann was submitted to University of North Texas, Health Science Center at Fort Worth, Texas, USA for analysis. This medical facility conducted a DNA analysis and comparison of the known sample of victim Madeleine McCann, and on 06 December 2007 it advised the results were a negative match to that of the body discovered near Galveston.

US law enforcement authorities have since determined the previously unidentified body that washed ashore near Galveston is that of deceased minor Riley Ana Sawyers. Both of the parents of Riley Ana Sawyers have since been formally charged with her murder.

Attached to this report is one photocopy of a DNA analysis report dated 06 December 2007 from the University of North Texas, Health Science Center at Fort Worth, Texas, USA entitled 'Missing Persons DNA Database Report'.

4358 to 4361 Information re Haplotype-S with rest results

TRANSLATION BY ALBYM

16-Processo 16; PDF page 4358
16_VOLUME_XVIa_Page_4358
16_VOLUME_XVIa_Page_4359
16_VOLUME_XVIa_Page_4360
16_VOLUME_XVIa_Page_4361
Extract from a letter from INML to the Inquiry clarifying the Haplotype S conundrum:
61
===============================
Em resposta ao oficio de V. Exmo. acima referenciado, cumpre-nos informar que um perfil de ADN mitocondrial nao e exclusivo e objectivamente identificativo de uma determinada pessoa. Salienta-se que apos consulta da base de dados de sequencias da regiao controlo do ADN mitocondrial, EMPOP database, os resultados obtidos permitiram a identificacao de 8 haplotipos identicos ao haplotipo S (sem diferences), num universo de 3830 amostras do grupo populacional 'West Eurasia", que compreende as populacoes da Europa.

Junto se anexa a consulta efectuada para melhor esclarecimento
:

================================
[Rough translation]
In response to your letter I advise that a mitochondrial DNA profile is not an exclusive or objective identification of a single [determined] person. Consulting the regional control sequences in the EMPOP database we find 8 results that are identical (zero differences) to the Haplotype S sample, from a total population [universe] of 3830 samples in the 'West Eurasia' population group, known as the populations of Europe.

See the printed consultation below:
============================

 
Input check

Sample Identification                   Perfil S                       
Database Release                        1                       
Maximum differences displayed           5                       
Include partially overlapping profiles  no                       
Consider(multiple)insertions in the
polyC-stretches at 16193, 309 and 573
individual differences as:
Selected ranges                         73-340       
                                        16024-16365                             

mtDNA query profile                     73A>G
                                        263A>G
                                        315.1C
                                        16256C>T
                                        16270C>T

Source                                  Forensic data                               

Selected populations                    Africa: ALL
                                        East Asia: ALL
                                        South East Asia: ALL
                                        West Eurasla: ALL

OUTPUT
Selected ranges: 73-340
                 16024-16365

mtDNA query profile: 73A>G, 263A>G, 315.1C, 16256C>T, 16270C>T



OVERALL RESULT SUMMARY (4527/4527)

DIFFERENCES TO    NUMBER OF   CUMULATIVE
QUERY PROFILE     HAPLOTYPES  NUMBER OF HAPLOTYPES
0                 8           8
1                 40          48
2                 54          102
3                 178         280                               
4                 442         722
5                 595         1317
>5                3210        4527

Africa RESULT SUMMARY (348/348)

East Asia RE5ULT SUMMARY (162/162)

South East Asia RESULT 5UMMARY (187/187)

West Eurasia RESULT SUMMARY (3830/3830)

DIFFERENCES TO    NUMBER OF   CUMULATIVE
QUERY PROFILE     HAPLOTYPES  NUMBER OF HAPLOTYPES
0                 8           8
1                 39          47
2                 54          101
3                 178         279
4                 429         708
5                 584         1292
>5                2538        3830
 
http://www.empop.org 19-05-2008

As I understand it, the EMPOP database is the global repository of regional profiles of mitochondrial DNA (mtDNA).
The person doing the query entered the specific mt DNA characteristics of JT's hair into the query form and found 8 exact matches in the database, i.e. at least eight maternal lines having the identical Haplotype to JT exist in Europe.
There were no exact matches in the other three regional groups.

Because of this result the inquiry could not say with absolute certainty that the hair found in Burgau actually came from JT because it could have come from any person born of a mother belonging to any of the eight profiles in the database - one profile, of course, had to be of JT's maternal line, so it could have been some distant (or close) relative.
13 - Fax re examination of Aparthotel Sol, C, in Burgau 2007.05.11
also Processo 05 page 1196
  TRANSLATED BY INES
01-01-Outros Apensos I, Volume I Page 13
05  Processo 05 Page  1196
 05_VOLUME_Va_Page_1196
01_volume_I_o_apenso_I_Page_13


Policia Judiciaria


FAX

Date: 15th May 2007

Classification: Urgent

To: The Director of the Scientific Police Laboratory

From: Chief Inspector Tavares de Almeida

Subject: NUIPC 201-070 GALGS


As it is necessary for the investigation of the NUIPC mentioned above into the disappearance of Madeleine McCann we ask you to determine the sense of carrying out an examination of the following residence:

Aparthotel Sol e Mar 2 Apartment C - Burgau.

We also request the preservation and respective processing of the vestiges collected there.

With compliments


Signed

Chief Inspector Tavares de Almeida

 

67 - Service information re collection of vestiges from 5A and Renault Scenic

Translated by Ines

Outros Apensos I, Vol 1  Page 67
01_volume_I_o_apenso_I_Page_67

Date: 2007-08-01


Service Information

To: The Coordinator of the Criminal Investigation, Dr Amaral

From: Inspector Joao Direito


Subject: Collection of eventual vestiges


I bring to your knowledge and following superior orders that today at about 20.00 the Assistant Specialists from the LPC Fernando Viegas and Lino Rodrigues, after seeing the recorded images from the sniffer dog inspection carried out on 31-07-2007 in Apartment 5 A of the Ocean Club Resort, duly explained in the report adjoining the inspection, proceeded to collect the tiles where the dogs used in the exercise signalled the eventual existence of blood vestiges as well as the presence of a cadaver in this same site.

The tiles in question were taken from the flooring with the aim of preserving the eventual vestiges existing there, in order to be subjected for competent laboratory examination.

The procedures were filmed in their totality with the aim of illustrating the way in which the lifting of the tiles was done and the tools that were used, thereby enabling forensic scientists to have a better perception of the circumstances inherent to the action taken. This resulted in the recording of two video cassettes (mini Dv) which are attached.

The LPC officers will prepare the corresponding report.



Signed

Inspector Direito

101 to 103 FSS report re DNA from Amelie & Sean McCann (English)
01-01-Outros Apensos I, Volume I Page 101 to 103
01_volume_I_o_apenso_I_Page_101

01_volume_I_o_apenso_I_Page_102

01_volume_I_o_apenso_I_Page_103

154 Delivery of mouth swabs for Tanner, Oldfield, G. McCann, and K. Healy 2007.05.10
01-01-Outros Apensos I, Volume I Page 154
01_volume_I_o_apenso_I_Page_154


Delivery Notice of Samples Collected at the Crime Scene

Date of delivery : 15th May 2007

Delivered to: INML

Date and time of collection: 10th May 2007

Place of collection: Portimao DIC

Samples delivered:


Mouth swabs from Jane Tanner
Mouth swab from Matthew Oldfield
Mouth swab from Gerald McCann
Mouth swab from Kate Healy

Observations: nothing to register
Lisbon 15th May 2007

284 to 299 Comparative analysis report of biological vestiges collected from various locales 2008.03.31
01-02 -OUTROS APENSOS I - volume II pages 284-299
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GENETIC & BIOLOGICAL FORENSIC SERVICES

 

Processo no. 2007/000565/PT-B
Processo no. 2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BC1



EXPERT REPORT

IDENTIFICATION OF BIOLOGICAL VESTIGES [remains] AND COMPARATIVE STUDY WITH REFERENCE SAMPLES

INFORMATION


REQUESTING ENTITY:
Department of Criminal Investigation of Portimao of the Pollcia Judiciaria and National Directorate.

PROCESSO: NUIPC 201/07.0GALGS
NAME: MADELEINE BETH MCCANN.

OFFICIAL LETTER N.' 003610, dated 31/10/2007, that solicited the performance of lawful examinations of various samples of hair recovered from the following locations:

- Apartment C, 2' Floor, of Aparthotel Sol e Mar, in Burgau
- Vehicle type Audi, model A4, licence-plate 10-91-PF
- Vehicle type Renault, model Kangoo, licence-plate 07-20-UI
- Vehicle type Volkswagen, model Passat, licence-plate 57-12-HP
- Sofas located in in the garage of the CID Portimao
- Garden of apartment 5A, block A, tourist resort "OCEAN CLUB", Praia da Luz, Lagos.

OFFICIAL LETTER N.' 004080, dated 12/12/2007, that solicited "examinations as appropriate, with the view to determine the nature of the mark (found on the counterpane [quilt/bedspread] of one of the beds in apartment 5A of the tourist resort OCEAN CLUB in Praia da Luz) and if it permits the identification of the profile indicated by letter (L)".

OFFICIAL LETTER N.' 004079, dated 12/12/2007, that says "to send an item marked as fibre and a plastic item both extracted from the car boot..., material already examined..., soliciting the indulgence of the reader in the sense that further examinations are necessary on the aforementioned material, using a method considered adequate for the detection of possible biological remains that may not have been detected in the first examination to which they were subjected."

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GENETIC & BIOLOGICAL FORENSIC SERVICES

Processo no. 2007/000565/PT-B
Processo no. 2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BC1


OFFICIAL LETTER No. 000550, dated de 04/07/07, that says to send 12 oral swabs [swatches] and hair collections, accompanied by respective letters of authorisation for their collection duly prepared and signed in the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria in respect of the following people: Silvia Maria Correia Ramos Baptista (head-hair only), Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras e Vitor Manuel Martins.

OFFICIAL LETTER No. 00551, dated 04/07/07, that says to send two oral swabs [swatches] and two capillary collections, obtaine from Michaela Walczuch e de Luis Filipe Duarte Antonio, accompanied by statements of swab collection and capillary collection duly prepared and signed in the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria.

MATERIAL RECEIVED

In this 2nd fase, 187 hairs [generic], 2 pieces from the car boot and reference samples from 15 individuals were received.

In the 1st fase, were received 258 vestigios and 12 reference samples - oral swabs [swatches] from 11 individuals. The vestigios included 257 hairs [generic] and one speck detected in a cloth fragment collected from the counterpane of the bed next to the window of the bedroom of the children in apartament 5A (envelope 5), as mentioned in the previous report.

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Processo no. 2007/000565/PT-B
Processo no. 2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BC1

DESCRIPTION OF THE MATERIAL RECEIVED

VESTIGIOS delivered by Policia Judiciaria on 08/11/2007:


With respect to Aparthotel Sol e Mar, 2', apartment C - Burgau

- 8 labelled envelopes with delivery note no. 420A/2007-CR/L:
Vestigio:
Vg1B- "Sundry head-hair collected from the kitchen floor of the apartment" - 20 hairs.
Vg2 - "Sundry head-hair collected at the entrance of the apartment" - 23 hairs.
Vg3 - "Sundry head-hair collected from the bedroom floor of the apartment" - 27 hairs.
Vg4 - "Sundry head-hair collected from the bed in the bedroom of the apartment" - 17 hairs.
Vg5 - "Sundry head-hair collected from the bathroom of apartment" - 16 hairs.
Vg6 - "Sundry head-hair collected from the floor of lounge of apartment" - 43 hairs.
Vg7 - "Sundry head-hair collected from a sofa presently in the lounge of the apartment"- 3 hairs.
Vg8 - "Sundry head-hair collected from a sofa presently in the lounge of the apartment"- 3 hairs.

With respect to Vehicle Audi A4 10-91-PF:

- 1 labelled envelope with delivery note no. 420C/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the front seat, right side"- 3 hairs.

With respect to Vehicle Renault Kangoo 07-50-UI:

- 3 labelled envelopes with delivery note no. 420E/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the row of seats at the rear of the Vehicle"- 1 hair.
Vg2 - "Sundry head-hair collected from the small area at the rear of Vehicle" - 2 hairs.
Vg3 - "Sundry head-hair collected from the front seat, right side" - 1 hair.

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Processo no. 2007/000565/PT-B
Processo no. 2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BC1


With respect to Vehicle Volkswagen Passat 57-12-HP:

- 1 labelled envelope with delivery note no. 420F/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the rear seat" - 6 hairs.

with respect to the sofas that are in the garage of the DIC Portimao:

- 3 labelled envelopes with delivery note no. 420B/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the sofa having one seat"- 7 hairs.
Vg2 - "Sundry head-hair collected from the sofa having two seats"- 2 hairs.
Vg3 - "Sundry head-hair collected from the sofa having one seat" - 2 hairs.

With respect to the garden area of apartment 5A, tourist resort "OCEAN CLUB", Praia da Luz, Lagos:

- 1 labelled envelope with delivery note no. 420D/2007-CR/L:
Vestigio:
Vg25 - "Sundry head-hair" - 11 hairs.

VESTIGIOS delivered by Policia Judiciaria on 14/12/2007:

With respect to the car boot:

- Pieces that included a fibre and plastic, having been previously analysed in various areas.

REFERENCE SAMPLES delivered by Policia Judiciaria on 04/07/2007:

- 14 oral swabs [swatches] and collections of head-hair, relating to:

Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras, Vltor Manuel Martins, Michaela Walczuch e Luis Filipe Duarte Antonio.

- Collection of head-hair relatiing to:

Silvia Maria Correia Ramos Baptista.

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Processo no. 2007/000565/PT-B
Processo no. 2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BC1

METHODOLOGY

1- Morphologic Analysis of the hairs


- Macro- and microscopic characterisation.

2- Nature of the sample

Phadebas Forensic test, for detection of saliva on the fragment of cloth corresponding to vestigio n'. 5 collected from the counterpane of the bed next to the window of the children's bedroom.

3- DNA Extraction:

DNA was extracted from the material received for analysis using one of the foloowing methods:

- Tissue and Hair Extraction kit-"DNA IQ(TM) System" (Promega)
- "QIAamp micro DNA kit" (Qiagen)
- Metodo de Chelex [1]

[1] Walsh P.S., Metzer D.A., Higuchi (1991). Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.

4- Study of the nuclear DNA

After extraction the following genetic markers were studied using PCR:

- Autosomic STRs: D8S1179, D21Sl l, D7S820, CSFIPO, D3S1358, HUMTH01, D13S317, D16S539, D2S1338, D19S433, HUMVWA31/A, TPOX, D18S51, D5S818, HUMFIBRA/FGA and Amelogene, using primers and amplification conditions recommended in kit AmpFISTR(TM) Identifiler (Applied Bioystems).

- Autosomic STRs: D3S1358, HUMTH01, D21SII, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSFIPO, Penta D, HUMVWA31/A, D8S1179, TPOX, HUMFIBRA/FGA, and Amelogene using primers and amplification conditions recommended in kit PowerPlex(TM) 16 System (Promega).

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GENETIC & BIOLOGICAL FORENSIC SERVICES

Processo no. 2007/000565/PT-B
Processo no. 2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BCI

5 - Estudo do DNA mitocondrial


The mitochondrial DNA study (HVRI e HVRII) was performed in accordance with the following metodology:

PCR Amplification:
PCR amplification of the HVI e HVII regions of the human mtDNA, using specified primers amplification conditions in accordance with Vigilant et al., 1989 [2], Wilson et al., 1995 [3] e Alvarez-lglesias et al., [4].

[2] Vigilant L, Pennington R. Harpending H. Kocher TD, Wilson AC. ]989. "Mitochondrial DNA Sequences in Single Hairs from a Southern African Population". Proc Natl Acad Sci, USA, 1989, Vol. 86: 9350 - 9354.
[3] Wilson MR, DiZinno JA, Polanskey D, Replogle J. Budowle B. 1995. "Validation of Mitochondrial DNA Sequencing for Forensic Casework Analysis". Int J Legal Med. 1995, 108: 68 - 74.
[4] Alvarez-lglesias V, Jaime JC, Carracedo A, Salas A.2007. "Coding region mitochondrial DNA SNPs:Targeting East Asian and Native American haplogroups" Forensic Sci. Int. Genetics, 2007, 1 :44-55.

Sequenciation: Direct sequenciation of the HVI e HVII segments by capillary electrophoresis in the automatic sequenciator "ABI PRISM 3100 ou 3130 Genetic Analyser" (Applied Biosystems) with detection by fluorescence, using one of the kits:

- "BigDye(R) Terminator vl.1 Cycle Sequencing" (Applied Biosystems)
- "BigDye(R) Terminator v1.3 Cycle Sequencing" (Applied Biosystems)
- "dRhodamine Terminator Cycle Sequencing Ready Reaction" (Applied Biosystems)

The program "SeqScape(R) Software Version 2.0" (Applied Biosystems) was used to compare the sequences obtained from the analysed samples with the CRS (Cambridge Reference Sequence).

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SERVIOS DE GENETICA E BIOLOGIA FORENSE

Processo no. 2007/000565/PT-B
Processo no. 2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BC1

RESULTS

[b]1- Morphologic analysis of the hairs


All the hairs received for analysis were subjected to macro- and microscopic characterisation. It was observed that some hairs were only stems while other had both root and stem. The majority of those with roots were found to be telogenic.

2- Nature of the sample

Phadebas Forensic test, for detection of saliva on the fragment of cloth corresponding to vestigio n'. 5 collected from the counterpane of the bed next to the window of the children's bedroom:
Positive.

3- Study of the nuclear DNA

Autosomic STRs results were obtained from the following vestigios: root of one of the hairs collected from the bedroom floor of the Burgau apartment (Vg3) and in the lower zone of te fibre from the car boot. Table 1 includes, also, the profile of the spot from the counterpane, already sent previously.

Table 1 - Autosomic STRs of vestigios.
 
                  From the bedroom       Vestigios inferior       Spot on the cloth
SYSTEMS           floor- Apart. in       zone of the fibre        fragment from the
                  Burgau -Vg 3           from the car boot        counterpane
Amelogenina           XY                        XY                      XY
D8S1179             13-15                     10-11                   12-13
D21S11              28-30                      29                    29-32.2
D7S820               9-10                     10-12                   10-12
CSFIPO              10-11                     10-13                   10-11
D3S1358             15-18                     14-16                   15-18
HUMTH01              6-9.3                     9-9.3                     9.3
D13S317                11                     11-12                   12-14
D16S539             11-12                     10-14                      11
D2S1338                17                     18-24                   18-25
D19S433                15                     13-14                   13-15
HUMVWA31/A          18-19                     17-18                      18
TPOX                 8-11                        8                       11
D18S51              16-18                     13-14                   17-18
D5S818                 12                     11-13                   12-13
HUMFIBRA/FGA           20                     21-25                   20-24
Penta E                                                                7-8
Penta I>                                                                 9
7
===================================
Translation:

GENETIC AND BIOLOGICAL FORENSIC SERVICES

Processo no. 2007/0005651PT-B
Processo no. 2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BCI

Autosomic STRs were also obtained relating to reference samples from:

Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras, Vitor Manuel Martins, Michaela Walczuch, Luis Filipe Duarte Antonio e Silvia Maria Correia Ramos Baptista.

4- Study of mitochondrial DNA

The vestigios delivered on 08/11/2007 and on 14/12/2007:

Mitochondrial DNA results were obtained in 137 hairs (roots and/or stems) from the 187 analysed. It was not possible to obtain any results from 46 hairs, and in 4 cases incomplete sequences were obtained.

5- Total of the samples studied:

Since May 2007, the following samples were analysed in the GENETIC AND BIOLOGICAL FORENSIC SERVICES offices of the North, Central e South Delegations of the Instituto Nacional of Medicina Legal:

- 25 reference samples.
- 447 vestigios, comprising:
>> 444 hairs, of which 432 were human;
>> 1 mark on a counterpane;

>> 2 objects from a car boot.

Of the 432 human hairs (roots and/or stems), 315 provided the results reflected in Table 2.


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Processo no. 2007/000226/LX-BC1

Tabela 2. Haplotypes of Mitochondrial DNA of the vestigios and comparison with reference samples

Haplo- No. of   Collection point of vestigios                 Comparison with
types  samples                                                reference samples
 B       4       3 Entrance hall Ocean(Vg7)
                 1 Entrance hall Burgau(Vg2)

 B1      1       1 Entrance hall Ocean(Vg7)                   Irene Ferro (IF)

                13 Lounge floor Ocean(Vg6)
                14 Entrance hall Ocean(Vg7)
 C      42       3 Bedroom entrance Ocean(Vg1)                Kate Healy (KH) and
                 1 Child's bed Ocean(Vg3)                     Jose Roque (JMBR)
                 4 Floor next to bed next  window Ocean(Vg4)
                 7 Floor next to child's  bed Ocean(Vg2)

 C*     11       1 Entrance hall Ocean(Vg7)
                10 Bedroom entrance Ocean(Vg1)

 D       1       1 Lounge floor Ocean(Vg6)

 D1      2       1 Bedroom entrance Ocean(Vg1)
                 1 Floor next to bed next window Ocean(Vg4)

 E       1       1 Entrance hall Ocean(Vg7)

 Ea      1       1 Sofa 1-seat; CID garage(Vg1)

 F       3       1 Lounge floor Ocean(Vg6) and               Vitor Martins (VM)
                 2 Entrance hall Ocean(Vg7)                  Vitor Martins (VM)

 G       1       1 Lounge floor Ocean(Vg6)                   Matthew Oldfield (MO)

 I       1       1 Entrance hall Ocean(Vg7)                  David Payne (DP)

                 1 Floor next to bed next window Ocean(Vg4)
 J       3       1 Entrance hall Ocean(Vg7)
                 1 Floor next to child's bed Ocean(Vg2)

 J1      0                                                   Ant6nio Duarte(ACD)

 K      16       1 Residencia Liliana(Vg1)
                13 vehicle Volkswagen Envl(3), Env3(5),      Michaela Walczuch (MW)
                   Env4(2), Env5(1), Env6(2)                 and Luis Ferro (LFMF)
                 1 Renault(Vg3) e 1 Passat(Vg1)
 
9
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Processo no.2007/000244/CR-B5,B6
Processo no.2007/000226/LX-BC1

Tabela 2. Haplotypes of DNA Mitocondrial dos vestigios e comparacao com as samples of referencia enviadas (cont.)
Haplo- No. of   Collection point of vestigios                      Comparison with 
types  samples                                                     reference samples

 L        4      1 Mark counterpane bed (saliva)(env5)
                 3 Floor next to bed next window Ocean(Vg4)

 La       4      1 Bedroom entrance Ocean(Vgl)
                 2 Entrance hall Ocean(Vg7)
                 1 Floor next to bed next window Ocean(Vg4)

 L2       1      1 Vehicle Volkswagen (Env5)

 M       27     13 Vehicle Volkswagen Envl(8), Env2(1), Env3(2), Env4(2)
                13 Residencia Liliana(Vgl)
                 1 Bathroom Burgau(Vg5)                             Robert Murat (RQMU)

 M*      22     22 Residencia Liliana(Vgl)

                 2 Lounge floor Ocean(Vg6)
                10 Entrance hall Ocean(Vg7)
 N       20      5 Bedroom entrance Ocean(Vgl)                      Gerald McCann (GM)
                 1 Floor next to bed next window Ocean(Vg4)
                 2 Floor next to child's bed Ocean(Vg2)

 N*       4      1 Bedroom entrance Ocean(Vgl), 2 next window Ocean(Vg4)
                 1 Corridor Ocean(Vg7)

 O        2      1 Lounge floor Ocean(Vg6), 1 entrance hall Ocean(Vg7) Russell O'Brien(RJB)

 P        2      2 Garden Ocean(Vg5)

 Q        2      1 Entrance hall Ocean(Vg7), 1 lounge floor Ocean(Vg6)

 R        0                                                         Nelson Costa (NFPC)

 R*       1      1 Vehicle Volkswagen Env4

 S        2      1 Bathroom (Vg5) Burgau and 1 Sofa (Vg7) Burgau    Jane Tanner (JT)

 T        8      1 LoungeFloor(Vg6), 2 Kitchen(Vgl),
                 2 EntHall(Vg2) Burgau, 3 BedFloor(Vg3) Burgau

 Ta       2      1 Fl Kitchen(Vgl) and 1 Bedroom Floor(Vg3) Burgau

 U        2      1 Entrance hall(Vg2) Burgau and 1 bed(Vg4) Burgau
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Tabela 2. Haplotypes of DNA Mitocondrial dos vestigios e comparacao com as samples of referencia enviadas (cont.)
Haplotypes    No. of    Collection point of vestigios             Comparison with
             samples                                              reference samples

    V           1       1 Lounge floor Ocean(Vg6)

    V*          1       1 Renault(Vg2)

    X           2       1 Bedroom entrance Ocean(Vgl) and 1 sofa 1-seat; garage(Vgl)

    Y           2       1 sofa 1-seat; garage(Vgl) and 1 corridor Ocean(Vg7)

    Z           3       l Residencia Liliana(Vgl)
                        2 sofa 1-seat garagem(Vgl e Vg3)

                        17 Ent.Hall(Vg2), 17 Kit.Floor(Vgl), 20 BedroomFloor(Vg3)
   AA          85       Brg                             
                        13 LoungeFl(Vg6), 4 bathroom(Vg5),10 Bed&bedroom(Vg4)
                        Burgau
                        4 Sofa(Vg7 e 8) Burgau

   AAa          6       3 Bed&bedroom(Vg4) and 3 bathroom(Vg5) Burgau

   AA*          9       7 LoungeFloor(Vg6) Burgau
                        2 Vehicle Passat(Vgl)

   AB           2       2 Audi A4(Vgl)
 
15 Haplotypes different from each other and from those above:
1    Bedroom entrance Ocean(Vgl)                                          .
1    Bed, main bedroom Burgau(Vg4)
1    Sofa of 1-seat; garage(Vgl)
1    Floor next to bed next to window Ocean(Vg4)
1    Sofa of 2-seats; garage(Vg2)
1    Entrance hall Burgau(Vg2)
1    Bathroom Burgau(Vg5)
1    Sofa of 2-seats; garage(Vg2)
1    Bedroom floor Burgau(Vg3)
1    Entrance hall Ocean(Vg7)
1    Lounge floor Ocean(Vg6)
1    Garden Ocean(Vg25)
1    Vehicle Volkswagen (Env5)
1    Bathroom Burgau(Vg5)
1    Entrance hall Ocean(Vg7)>/span>
a Difference in 309.1C with respect to the corresponding Haplotype
* Results obtained solely in HVI
1 One difference in regio HVII with respect to the corresponsing Haplotype
2 Two differences in regio HVII with respect to the corresponsing Haplotype

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GENETIC AND BIOLOGICAL FORENSIC SERVICES

Processo no. 2007/000565/PT-B
Processo no.2007/000244/CR-B5,B6
Processo no.2007/000226/LX-BC1

CONCLUSIONS


First: In total in the two phases of the case there were 25 reference samples and 447 vestiges studied: 1 spot on the counterpane; 2 objects from the car boot and 444 hairs. The macro- and microscopic analyses of the 444 hairs revealed that 432 were humano and 12 non-human, no result being obtained for 98 hairs and only partial results for l9 hairs. With respect to the plastic object from the car boot no results were obtained.

Second: The search for
saliva in the mark on the counterpane from one of the beds in apartament 5A of tourist resort Ocean Club was positive.

Third: The study of autosomic STRs from the sample received in the 2 phases revealed:

There were matches between the reference sample genetic profiles and the following analysed samples:

- hair root (CEnv 7-51) collected in apartament Ocean Club (entrance hall env.7) and Gerald Mccann, father of the victim.

- hair root (PVT07) collected in the Volkswagen 44-77-KD (floor in front of rear seats env.4) and Robert James Queriol Eveleight Murat.

- hair root (C742-05) collected in Volkswagen 44-77-KD (floor in front of front seats - env.3) and Michaela Walczuch.

There were no matches between the reference sample genetic profiles and the following analysed samples:

- Mark on the cloth fragment collected from apartament Ocean Club (counterpane of the bed next to the window of the children's bedroom - env. 5).

- Hair root collected in the apartament in Burgau (bedroom floor - vg3).

- Vestigios in the lower zone of the fibre from the car boot.

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GENETIC AND BIOLOGICAL FORENSIC SERVICES

Processo no. 2007/000565/PT-B
Processo no.2007/000244/CR-B5,B6
Processo no.2007/000226/LX-BC1

Fourth: The mitochondrial DNA study revealed (a):


In the analysed samples from apartament 5-A of the Ocean Club:

- The Haplotype identified by the letter B1, present in one sample, and identical to that of Maria Irene Trovao Ferro (IF), meaning that sample was from her or an individual of the same maternal bloodline.

- The Haplotype identified by the letters C e C*, present in 53 samples, and identical to that of Kate Healy (KH), mother of the victim and to that of Jose Maria Batista Roque (JMBR), meaning those sample were from those persons or individuals of the same maternal bloodlines.

- The Haplotype identified by the letter F, present in 3 samples, and identical to that of Vltor Manuel Martins (VM), meaning those samples were from that person or individuals of the same maternal bloodlines.

- The Haplotype identified by the letter G, present in one sample, and identical to that of Matthew David Oldfield (MO), meaning that sample was from that person or an individual of the same maternal bloodline.

- The Haplotype identified by the letter I, present in one sample, and identical to that of David Anthony Payne (DP), meaning that sample was from that person or an individual of the same maternal bloodline.

- The Haplotype identified by the letters N e N*, present in 24 samples, and identical to that of Gerald Mccann (GM), father of the victim, meaning those sample were from that person or individuals of the same maternal bloodlines.

- The Haplotype identified by the letter O. present in 2 samples, and identical to that of Russell James O'Brien (RJB), meaning those samples were from that person or individuals of the same maternal bloodlines.

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GENETIC AND BIOLOGICAL FORENSIC SERVICES

Processo no.2007/000565/PT-B
Processo no.2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BC1


In the analysed samples from other locations:

- The Haplotype identified by the letter K, present in 16 samples, (1 in the Residencia Liliana, 13 in vehicle Volkswagen, 1 in the Renault and l in the Passat) and identical to that of Michaela Walczuch (MW) and to that of Luis Filipe Monteiro Ferro (LFMF), meaning those samples were from those persons or individuals of the same maternal bloodlines.

- The Haplotype identified by the letters M e M*, present in 49 samples, (35 in the Residencia Liliana, 13 in the vehicle Volkswagen and 1 in the bathroom of the apartament in Burgau), and identical to that of Robert James Queriol Eveleight Murat (RQMU), meaning those samples were from that person or individuals of the same maternal bloodline.

- The Haplotype identified by the letter R*, present in one sample from the vehicle Volkswagen, and identical in HVI, to that of Nelson Filipe Pacheco da Costa (NFPC) - letter R, meaning that sample was from that person or an individual of the same amternal bloodline.

- The Haplotype identified by the letter S, present in 2 samples, (apartment in Burgau), and identical to that of Jane Michelle Tanner (JT), meaning those samples were from that person or individuals of the same maternal bloodline.

5a- The study of the mitochondrial DNA revealed also (a):

- The Haplotypes identified by letters D, D1, J, L, La, P e Q are different from those above, having been found solely in samples from apartament 5-A Ocean Club, not corresponding to any of the reference sample Haplotypes.

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Processo no.2007/000565/PT-B
Processo no.2007/000244/CR-B5,B6
Processo no.2007/000226/LX-BC1


- The Haplotypes identified by the letters L2, T, Ta, U e AB are also different from those above, having been found in the apartament in Burgau or in one of the vehicles, not corresponding to any of the reference sample Haplotypes:

Haplotype L2 - Vehicle Volkswagen
Haplotypes T, Ta - apartament in Burgau
Haplotype U - apartament in Burgau
Haplotype AB - Vehicle Audi A4

- The Haplotypes identified by the letters B, E, Ea, V, V*, X, Y, Z, AA, AAa e AA* are different from those above and distinct from the reference samples, having the same Haplotype been found in distinct locations:

Haplotype B - apart. Ocean Club and apart. Burgau
Haplotypes E, Ea - apart. Ocean Club and sofa of 1-seat in the garage in Portimao
Haplotypes V, V* - apart. Ocean Club and vehicle Renault
Haplotype X - apart. Ocean Club e sofa of 1-seat in the garage in Portimao
Haplotype Y - apart. Ocean Club e sofa of 1-seat in the garage in Portimao
Haplotype Z - Residencia Liliana e sofa of 1-seat in the garage in Portimao
Haplotypes AA, AAa, AA* - apartment in Burgau and vehicle Passat

- The Haplotype identified by the letter J1 corresponds to Antonio Conceicao Duarte (ACD).
This Haplotype was not identified in any of the analysed vestigios.
____________________________
(a) The Haplotypes C*, M*, N*, R*, V* e AA* are identical in the region HVI to the corresponding Haplotype, there not having been obtained any results in region HVIT.
The Haplotypes Ea. La, Ta e AAa presented a difference in 309.1C in relation to that of the corresponding Haplotype.
The Haplotypes Bl, Dl, Jl e Ll presented a difference in the region HVII in relation to that of the corresponding Haplotype.
The Haplotype L2 presented two differences in the region HVII in relation to that of the corresponding Haplotype.

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Processo no.2007/000565/PT-B
Processo no.2007/000244/CR-B5,B6
Processo no. 2007/000226/LX-BC1


- The remaining 15 analysed vestigios reflected 15 Haplotypes different from each other and distinct from all of the above(including the reference samples).

Instituto Nacional of Medicina Legal, 31 of Marco of 2008

[centre]Director da Delegacao do Centro e Vogal do Conselho Directivo

Prof. Doutor Francisco Corte Real

Directora of Service - Delegacao do Norte

Prof. Doutora Maria of Fatima Pinheiro

Directora of Service - Delegacao do Centro

Dra. Maria Joao Anjos Porto

Directora of Service - Delegacao do Sul

Dra. Rosa Maria Espinheira
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